Applications of high-throughput RNA interference screens to problems in cell and developmental biology.

Journal Article (Journal Article;Review)

RNA interference (RNAi) in tissue culture cells has emerged as an excellent methodology for identifying gene functions systematically and in an unbiased manner. Here, we describe how RNAi high-throughput screening (HTS) in Drosophila cells are currently being performed and emphasize the strengths and weaknesses of the approach. Further, to demonstrate the versatility of the technology, we provide examples of the various applications of the method to problems in signal transduction and cell and developmental biology. Finally, we discuss emerging technological advances that will extend RNAi-based screening methods.

Full Text

Duke Authors

Cited Authors

  • Perrimon, N; Mathey-Prevot, B

Published Date

  • January 1, 2007

Published In

Volume / Issue

  • 175 / 1

Start / End Page

  • 7 - 16

PubMed ID

  • 17244760

Pubmed Central ID

  • PMC1775003

International Standard Serial Number (ISSN)

  • 0016-6731

Digital Object Identifier (DOI)

  • 10.1534/genetics.106.069963


  • eng

Conference Location

  • United States