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High-throughput RNA interference screens in Drosophila tissue culture cells.

Publication ,  Journal Article
Armknecht, S; Boutros, M; Kiger, A; Nybakken, K; Mathey-Prevot, B; Perrimon, N
Published in: Methods Enzymol
2005

This chapter describes the method used to conduct high-throughput screening (HTs) by RNA interference in Drosophila tissue culture cells. It covers four main topics: (1) a brief description of the existing platforms to conduct RNAi-screens in cell-based assays; (2) a table of the Drosophila cell lines available for these screens and a brief mention of the need to establish other cell lines as well as cultures of primary cells; (3) a discussion of the considerations and protocols involved in establishing assays suitable for HTS in a 384-well format; and (A) a summary of the various ways of handling raw data from an ongoing screen, with special emphasis on how to apply normalization for experimental variation and statistical filters to sort out noise from signals.

Duke Scholars

Published In

Methods Enzymol

DOI

ISSN

0076-6879

Publication Date

2005

Volume

392

Start / End Page

55 / 73

Location

United States

Related Subject Headings

  • Transfection
  • Tissue Culture Techniques
  • RNA Interference
  • Genes, Reporter
  • Drosophila
  • Cell Line
  • Biochemistry & Molecular Biology
  • Animals
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
 

Citation

APA
Chicago
ICMJE
MLA
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Armknecht, S., Boutros, M., Kiger, A., Nybakken, K., Mathey-Prevot, B., & Perrimon, N. (2005). High-throughput RNA interference screens in Drosophila tissue culture cells. Methods Enzymol, 392, 55–73. https://doi.org/10.1016/S0076-6879(04)92004-6
Armknecht, Susan, Michael Boutros, Amy Kiger, Kent Nybakken, Bernard Mathey-Prevot, and Norbert Perrimon. “High-throughput RNA interference screens in Drosophila tissue culture cells.Methods Enzymol 392 (2005): 55–73. https://doi.org/10.1016/S0076-6879(04)92004-6.
Armknecht S, Boutros M, Kiger A, Nybakken K, Mathey-Prevot B, Perrimon N. High-throughput RNA interference screens in Drosophila tissue culture cells. Methods Enzymol. 2005;392:55–73.
Armknecht, Susan, et al. “High-throughput RNA interference screens in Drosophila tissue culture cells.Methods Enzymol, vol. 392, 2005, pp. 55–73. Pubmed, doi:10.1016/S0076-6879(04)92004-6.
Armknecht S, Boutros M, Kiger A, Nybakken K, Mathey-Prevot B, Perrimon N. High-throughput RNA interference screens in Drosophila tissue culture cells. Methods Enzymol. 2005;392:55–73.
Journal cover image

Published In

Methods Enzymol

DOI

ISSN

0076-6879

Publication Date

2005

Volume

392

Start / End Page

55 / 73

Location

United States

Related Subject Headings

  • Transfection
  • Tissue Culture Techniques
  • RNA Interference
  • Genes, Reporter
  • Drosophila
  • Cell Line
  • Biochemistry & Molecular Biology
  • Animals
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology