Rapid selection of tetracycline-controlled inducible cell lines using a green fluorescent-transactivator fusion protein.
Publication
, Journal Article
Callus, BA; Mathey-Prevot, B
Published in: Biochem Biophys Res Commun
April 21, 1999
We describe a modification of the tetracycline-controlled expression system that facilitates the rapid identification of tetracycline-sensitive clones. The TetR/VP16 transactivator protein was tagged with the green fluorescent protein (GFP) at its N-terminus. This results in a functional transactivator which allows cells expressing high levels of the modified transactivator to be selected by fluorescent-activated cell sorting. After expansion, single cell clones that maintain a high level of GFP fluorescence can be tested for their ability to transactivate a luciferase gene under control of the Tet operator, leading to the rapid identification of clones with strong inducibility.
Duke Scholars
Published In
Biochem Biophys Res Commun
DOI
ISSN
0006-291X
Publication Date
April 21, 1999
Volume
257
Issue
3
Start / End Page
874 / 878
Location
United States
Related Subject Headings
- Transfection
- Trans-Activators
- Time Factors
- Tetracycline
- Recombinant Fusion Proteins
- Promoter Regions, Genetic
- Mice
- Luminescent Proteins
- Luciferases
- Herpes Simplex Virus Protein Vmw65
Citation
APA
Chicago
ICMJE
MLA
NLM
Callus, B. A., & Mathey-Prevot, B. (1999). Rapid selection of tetracycline-controlled inducible cell lines using a green fluorescent-transactivator fusion protein. Biochem Biophys Res Commun, 257(3), 874–878. https://doi.org/10.1006/bbrc.1999.0558
Callus, B. A., and B. Mathey-Prevot. “Rapid selection of tetracycline-controlled inducible cell lines using a green fluorescent-transactivator fusion protein.” Biochem Biophys Res Commun 257, no. 3 (April 21, 1999): 874–78. https://doi.org/10.1006/bbrc.1999.0558.
Callus BA, Mathey-Prevot B. Rapid selection of tetracycline-controlled inducible cell lines using a green fluorescent-transactivator fusion protein. Biochem Biophys Res Commun. 1999 Apr 21;257(3):874–8.
Callus, B. A., and B. Mathey-Prevot. “Rapid selection of tetracycline-controlled inducible cell lines using a green fluorescent-transactivator fusion protein.” Biochem Biophys Res Commun, vol. 257, no. 3, Apr. 1999, pp. 874–78. Pubmed, doi:10.1006/bbrc.1999.0558.
Callus BA, Mathey-Prevot B. Rapid selection of tetracycline-controlled inducible cell lines using a green fluorescent-transactivator fusion protein. Biochem Biophys Res Commun. 1999 Apr 21;257(3):874–878.
Published In
Biochem Biophys Res Commun
DOI
ISSN
0006-291X
Publication Date
April 21, 1999
Volume
257
Issue
3
Start / End Page
874 / 878
Location
United States
Related Subject Headings
- Transfection
- Trans-Activators
- Time Factors
- Tetracycline
- Recombinant Fusion Proteins
- Promoter Regions, Genetic
- Mice
- Luminescent Proteins
- Luciferases
- Herpes Simplex Virus Protein Vmw65