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Cooperation between core binding factor and adjacent promoter elements contributes to the tissue-specific expression of interleukin-3.

Publication ,  Journal Article
Taylor, DS; Laubach, JP; Nathan, DG; Mathey-Prevot, B
Published in: J Biol Chem
June 14, 1996

Tissue-specific expression of interleukin-3 (IL-3) is mediated via cis-acting elements located within 315 base pairs of the transcription start. This is achieved in part through the positive activities of the AP-1 and Elf-1 sites in the IL-3 promoter. The contribution to T cell-specific expression by other promoter sites was assessed in a transient expression assay with IL-3 promoter constructs linked to a luciferase gene, focusing initially on the core binding factor (CBF) site, which is footprinted in vivo upon T cell activation. Activity of the CBF site is shown to be critically dependent on the adjacent activator site Act-1. Together the Act-1 and CBF sites form a functional unit (AC unit) with dual activity. The AC unit is demonstrated to enhance basal activity of promoters both in fibroblasts and T cells. This activity is further inducible in activated T cells, but not in fibroblasts. In addition to the already identified NIP repressor site, evidence is presented for a second repressor region that restricts promoter activity in fibroblasts. Finally, a novel positive regulatory element has been mapped in the IL-3 promoter between nucleotide -180 and -210 that leads to increased expression in T cells. Together these results demonstrate that T cell expression of IL-3 is not specified by the activity of a single tissue-specific element, but instead involves multiple interacting elements that provide both specific positive regulation in T cells and specific negative regulation in fibroblasts.

Duke Scholars

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

June 14, 1996

Volume

271

Issue

24

Start / End Page

14020 / 14027

Location

United States

Related Subject Headings

  • Transfection
  • Transcription Factors
  • Transcription Factor AP-1
  • TATA Box
  • T-Lymphocytes
  • Sequence Homology, Nucleic Acid
  • Regulatory Sequences, Nucleic Acid
  • Recombinant Proteins
  • Promoter Regions, Genetic
  • Organ Specificity
 

Citation

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MLA
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Taylor, D. S., Laubach, J. P., Nathan, D. G., & Mathey-Prevot, B. (1996). Cooperation between core binding factor and adjacent promoter elements contributes to the tissue-specific expression of interleukin-3. J Biol Chem, 271(24), 14020–14027. https://doi.org/10.1074/jbc.271.24.14020
Taylor, D. S., J. P. Laubach, D. G. Nathan, and B. Mathey-Prevot. “Cooperation between core binding factor and adjacent promoter elements contributes to the tissue-specific expression of interleukin-3.J Biol Chem 271, no. 24 (June 14, 1996): 14020–27. https://doi.org/10.1074/jbc.271.24.14020.
Taylor DS, Laubach JP, Nathan DG, Mathey-Prevot B. Cooperation between core binding factor and adjacent promoter elements contributes to the tissue-specific expression of interleukin-3. J Biol Chem. 1996 Jun 14;271(24):14020–7.
Taylor, D. S., et al. “Cooperation between core binding factor and adjacent promoter elements contributes to the tissue-specific expression of interleukin-3.J Biol Chem, vol. 271, no. 24, June 1996, pp. 14020–27. Pubmed, doi:10.1074/jbc.271.24.14020.
Taylor DS, Laubach JP, Nathan DG, Mathey-Prevot B. Cooperation between core binding factor and adjacent promoter elements contributes to the tissue-specific expression of interleukin-3. J Biol Chem. 1996 Jun 14;271(24):14020–14027.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

June 14, 1996

Volume

271

Issue

24

Start / End Page

14020 / 14027

Location

United States

Related Subject Headings

  • Transfection
  • Transcription Factors
  • Transcription Factor AP-1
  • TATA Box
  • T-Lymphocytes
  • Sequence Homology, Nucleic Acid
  • Regulatory Sequences, Nucleic Acid
  • Recombinant Proteins
  • Promoter Regions, Genetic
  • Organ Specificity