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The murine DUB-1 gene is specifically induced by the betac subunit of interleukin-3 receptor.

Publication ,  Journal Article
Zhu, Y; Pless, M; Inhorn, R; Mathey-Prevot, B; D'Andrea, AD
Published in: Mol Cell Biol
September 1996

Cytokines regulate cell growth and differentiation by inducing the expression of specific target genes. We have recently isolated a cytokine-inducible, immediate-early cDNA, DUB-1, that encodes a deubiquitinating enzyme. The DUB-1 mRNA was specifically induced by the receptors for interleukin-3, granulocyte-macrophage colony-stimulating factor, and interleukin-5, suggesting a role for the beta common (betac subunit known to be shared by these receptors. In order to identify the mechanism of cytokine induction, we isolated a murine genomic clone for DUB-1 containing a functional promoter region. The DUB-1 gene contains two exons, and the nucleotide sequence of its coding region is identical to the sequence of DUB-1 cDNA. Various regions of the 5' flanking region of the DUB-1 gene were assayed for cytokine-inducible activity. An enhancer region that retains the beta c-specific inducible activity of the DUB-1 gene was identified. Enhancer activity was localized to a 112-bp fragment located 1.4 kb upstream from the ATG start codon. Gel mobility shift assays revealed two specific protein complexes that bound to this minimal enhancer region. One complex was induced by betac signaling, while the other was noninducible. Finally, the membrane-proximal region of human betac was required for DUB-1 induction. In conclusion, DUB-1 is the first example of an immediate-early gene that is induced by a specific subunit of a cytokine receptor. Further analysis of the DUB-1 enhancer element may reveal specific determinants of a betac-specific signaling pathway.

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Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

September 1996

Volume

16

Issue

9

Start / End Page

4808 / 4817

Location

United States

Related Subject Headings

  • Signal Transduction
  • Receptors, Interleukin-3
  • Protein Kinases
  • Molecular Sequence Data
  • Mice
  • Immediate-Early Proteins
  • Humans
  • Genes, Immediate-Early
  • Gene Expression Regulation
  • Enhancer Elements, Genetic
 

Citation

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Zhu, Y., Pless, M., Inhorn, R., Mathey-Prevot, B., & D’Andrea, A. D. (1996). The murine DUB-1 gene is specifically induced by the betac subunit of interleukin-3 receptor. Mol Cell Biol, 16(9), 4808–4817. https://doi.org/10.1128/MCB.16.9.4808
Zhu, Y., M. Pless, R. Inhorn, B. Mathey-Prevot, and A. D. D’Andrea. “The murine DUB-1 gene is specifically induced by the betac subunit of interleukin-3 receptor.Mol Cell Biol 16, no. 9 (September 1996): 4808–17. https://doi.org/10.1128/MCB.16.9.4808.
Zhu Y, Pless M, Inhorn R, Mathey-Prevot B, D’Andrea AD. The murine DUB-1 gene is specifically induced by the betac subunit of interleukin-3 receptor. Mol Cell Biol. 1996 Sep;16(9):4808–17.
Zhu, Y., et al. “The murine DUB-1 gene is specifically induced by the betac subunit of interleukin-3 receptor.Mol Cell Biol, vol. 16, no. 9, Sept. 1996, pp. 4808–17. Pubmed, doi:10.1128/MCB.16.9.4808.
Zhu Y, Pless M, Inhorn R, Mathey-Prevot B, D’Andrea AD. The murine DUB-1 gene is specifically induced by the betac subunit of interleukin-3 receptor. Mol Cell Biol. 1996 Sep;16(9):4808–4817.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

September 1996

Volume

16

Issue

9

Start / End Page

4808 / 4817

Location

United States

Related Subject Headings

  • Signal Transduction
  • Receptors, Interleukin-3
  • Protein Kinases
  • Molecular Sequence Data
  • Mice
  • Immediate-Early Proteins
  • Humans
  • Genes, Immediate-Early
  • Gene Expression Regulation
  • Enhancer Elements, Genetic