Multiple transcription start sites and 5' alternate splicing of murine IL-3 receptor beta-chain transcripts.


Journal Article

The murine interleukin-3 receptor beta-chain genes, IL-3R beta IL-3 and IL-3R beta c, encode the signal transducing chains of the high affinity receptors for IL-3 and IL-3, GM-CSF and IL-5 respectively. Little is known about the regulation of their expression. To enable the study of the promotors of IL-3R beta IL-3 and IL-3R beta c, we have characterized their respective 5' untranslated regions using a modified 5' RACE protocol. Four classes of alternatively spliced transcripts were isolated that initiate in a 400 nt region upstream from a previously reported start site(1). The initially reported partial IL-3R beta IL-3 clone belongs to the first class of transcripts(2). The second class starts in the middle of an intron as defined by the first class. The 3rd and 4th class establish 2 novel splice donor sites. These results were confirmed by RNAse-protection assay. The complex organization as evident from our data establishes an experimental framework for future experiments aimed at the study of the promoters for the murine IL-3R beta genes.

Full Text

Duke Authors

Cited Authors

  • Norga, K; Pless, M; Stanulla, M; TePas, EC; Mathey-Prevot, B

Published Date

  • November 30, 1994

Published In

Volume / Issue

  • 205 / 1

Start / End Page

  • 886 - 892

PubMed ID

  • 7999127

Pubmed Central ID

  • 7999127

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1006/bbrc.1994.2747


  • eng

Conference Location

  • United States