Skip to main content

Interleukin-3 expression by activated T cells involves an inducible, T-cell-specific factor and an octamer binding protein.

Publication ,  Journal Article
Davies, K; TePas, EC; Nathan, DG; Mathey-Prevot, B
Published in: Blood
February 15, 1993

Interleukin-3 (IL-3) is exclusively expressed by activated T and natural killer cells, a function that is tightly controlled both in a lineage-specific and in a stimulation-dependent manner. We have investigated the protein binding characteristics and functional importance of the ACT-1-activating region of the IL-3 promoter. This region binds an inducible, T-cell-specific factor over its 5' end, a site that is necessary for the expression of IL-3 in the absence of other upstream elements. Over its 3' end, it binds a factor that is ubiquitously and constitutively expressed. This factor is Oct-1 or an immunologically related octamer-binding protein, and it plays a role in coordinating the activity of several regulatory elements. These characteristics make the ACT-1 site analogous to the activating ARRE-1 site in the IL-2 promoter. Furthermore, and despite a lack of sequence homology, the promoters of IL-3 and IL-2 share an organizational pattern of regulatory elements that is likely to be important for the T-cell-specific expression of these genes.

Duke Scholars

Published In

Blood

ISSN

0006-4971

Publication Date

February 15, 1993

Volume

81

Issue

4

Start / End Page

928 / 934

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription Factors
  • T-Lymphocytes
  • Regulatory Sequences, Nucleic Acid
  • Promoter Regions, Genetic
  • Octamer Transcription Factor-1
  • Molecular Sequence Data
  • Lymphocyte Activation
  • Leukemia
  • Interleukin-3
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Davies, K., TePas, E. C., Nathan, D. G., & Mathey-Prevot, B. (1993). Interleukin-3 expression by activated T cells involves an inducible, T-cell-specific factor and an octamer binding protein. Blood, 81(4), 928–934.
Davies, K., E. C. TePas, D. G. Nathan, and B. Mathey-Prevot. “Interleukin-3 expression by activated T cells involves an inducible, T-cell-specific factor and an octamer binding protein.Blood 81, no. 4 (February 15, 1993): 928–34.
Davies K, TePas EC, Nathan DG, Mathey-Prevot B. Interleukin-3 expression by activated T cells involves an inducible, T-cell-specific factor and an octamer binding protein. Blood. 1993 Feb 15;81(4):928–34.
Davies K, TePas EC, Nathan DG, Mathey-Prevot B. Interleukin-3 expression by activated T cells involves an inducible, T-cell-specific factor and an octamer binding protein. Blood. 1993 Feb 15;81(4):928–934.

Published In

Blood

ISSN

0006-4971

Publication Date

February 15, 1993

Volume

81

Issue

4

Start / End Page

928 / 934

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription Factors
  • T-Lymphocytes
  • Regulatory Sequences, Nucleic Acid
  • Promoter Regions, Genetic
  • Octamer Transcription Factor-1
  • Molecular Sequence Data
  • Lymphocyte Activation
  • Leukemia
  • Interleukin-3