Interleukin-3 expression by activated T cells involves an inducible, T-cell-specific factor and an octamer binding protein.

Published

Journal Article

Interleukin-3 (IL-3) is exclusively expressed by activated T and natural killer cells, a function that is tightly controlled both in a lineage-specific and in a stimulation-dependent manner. We have investigated the protein binding characteristics and functional importance of the ACT-1-activating region of the IL-3 promoter. This region binds an inducible, T-cell-specific factor over its 5' end, a site that is necessary for the expression of IL-3 in the absence of other upstream elements. Over its 3' end, it binds a factor that is ubiquitously and constitutively expressed. This factor is Oct-1 or an immunologically related octamer-binding protein, and it plays a role in coordinating the activity of several regulatory elements. These characteristics make the ACT-1 site analogous to the activating ARRE-1 site in the IL-2 promoter. Furthermore, and despite a lack of sequence homology, the promoters of IL-3 and IL-2 share an organizational pattern of regulatory elements that is likely to be important for the T-cell-specific expression of these genes.

Full Text

Duke Authors

Cited Authors

  • Davies, K; TePas, EC; Nathan, DG; Mathey-Prevot, B

Published Date

  • February 15, 1993

Published In

Volume / Issue

  • 81 / 4

Start / End Page

  • 928 - 934

PubMed ID

  • 8428000

Pubmed Central ID

  • 8428000

International Standard Serial Number (ISSN)

  • 0006-4971

Language

  • eng

Conference Location

  • United States