Erythropoietin receptor signals both proliferation and erythroid-specific differentiation.

Published

Journal Article

Ectopic expression of the erythropoietin receptor (EPO-R) in Ba/F3, an interleukin 3-dependent progenitor cell line, confers EPO-dependent cell growth. To examine whether the introduced EPO-R could affect differentiation, we isolated Ba/F3-EPO-R subclones in interleukin 3 and assayed for the induction of beta-globin mRNA synthesis after exposure to EPO. Detection of beta-globin mRNA was observed within 3 days of EPO treatment, with peak levels accumulating after 10 days. When EPO was withdrawn, expression of beta-globin mRNA persisted in most clones, suggesting that commitment to erythroid differentiation had occurred. Although EPO-R expression also supports EPO-dependent proliferation of CTLL-2, a mature T-cell line, those cells did not produce globin transcripts, presumably because they lack requisite cellular factors involved in erythrocyte differentiation. We conclude that the EPO-R transmits signals important for both proliferation and differentiation along the erythroid lineage.

Full Text

Duke Authors

Cited Authors

  • Liboi, E; Carroll, M; D'Andrea, AD; Mathey-Prevot, B

Published Date

  • December 1, 1993

Published In

Volume / Issue

  • 90 / 23

Start / End Page

  • 11351 - 11355

PubMed ID

  • 8248252

Pubmed Central ID

  • 8248252

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.90.23.11351

Language

  • eng

Conference Location

  • United States