Expression of human interleukin-3 (multi-CSF) is restricted to human lymphocytes and T-cell tumor lines.

Journal Article

While the cellular sources for granulocyte-macrophage colony-stimulating factor (GM-CSF) are known to be widely distributed among several cell types, interleukin-3 (IL-3) gene expression has been demonstrated in only certain T-cell clones and in blood mononuclear cells stimulated with phytohemagglutinin (PHA) and phorbol-myristate-acetate (PMA). To determine which blood cells were responsible for this expression, we fractionated PHA/PMA-stimulated mononuclear cells and identified T lymphocytes as the source of IL-3 mRNA. Low-level IL-3 expression was detected as well in several stimulated human T-cell lines. Hematopoietic stromal cells such as fibroblasts and endothelial cells could not be induced to express IL-3 mRNA. The kinetics of IL-3 mRNA induction in mononuclear cells and lymphocytes stimulated with PHA/PMA or anti-CD3 monoclonal antibody (MoAb) and interleukin-1 (IL-1) were similar to those observed for GM-CSF expression.

Full Text

Duke Authors

Cited Authors

  • Niemeyer, CM; Sieff, CA; Mathey-Prevot, B; Wimperis, JZ; Bierer, BE; Clark, SC; Nathan, DG

Published Date

  • March 1989

Published In

Volume / Issue

  • 73 / 4

Start / End Page

  • 945 - 951

PubMed ID

  • 2645952

International Standard Serial Number (ISSN)

  • 0006-4971

Language

  • eng

Conference Location

  • United States