Expression of human interleukin-3 (multi-CSF) is restricted to human lymphocytes and T-cell tumor lines.
Journal Article (Journal Article)
While the cellular sources for granulocyte-macrophage colony-stimulating factor (GM-CSF) are known to be widely distributed among several cell types, interleukin-3 (IL-3) gene expression has been demonstrated in only certain T-cell clones and in blood mononuclear cells stimulated with phytohemagglutinin (PHA) and phorbol-myristate-acetate (PMA). To determine which blood cells were responsible for this expression, we fractionated PHA/PMA-stimulated mononuclear cells and identified T lymphocytes as the source of IL-3 mRNA. Low-level IL-3 expression was detected as well in several stimulated human T-cell lines. Hematopoietic stromal cells such as fibroblasts and endothelial cells could not be induced to express IL-3 mRNA. The kinetics of IL-3 mRNA induction in mononuclear cells and lymphocytes stimulated with PHA/PMA or anti-CD3 monoclonal antibody (MoAb) and interleukin-1 (IL-1) were similar to those observed for GM-CSF expression.
Full Text
Duke Authors
Cited Authors
- Niemeyer, CM; Sieff, CA; Mathey-Prevot, B; Wimperis, JZ; Bierer, BE; Clark, SC; Nathan, DG
Published Date
- March 1989
Published In
Volume / Issue
- 73 / 4
Start / End Page
- 945 - 951
PubMed ID
- 2645952
International Standard Serial Number (ISSN)
- 0006-4971
Language
- eng
Conference Location
- United States