Neuronal pp60c-src contains a six-amino acid insertion relative to its non-neuronal counterpart.

Published

Journal Article

Neuronal cells express a pp60c-src variant that displays an altered electrophoretic mobility and a different V8 peptide pattern relative to pp60c-src expressed in tissues of non-neuronal origin. To determine whether the neuronal form of pp60c-src is encoded by a brain-specific messenger RNA, a mouse brain complementary DNA (cDNA) library was screened with a chicken c-src probe and a 3.8-kilobase c-src cDNA clone was isolated. This clone encodes a 60-kilodalton protein that differs from chicken or human pp60c-src primarily in having six extra amino acids (Arg-Lys-Val-Asp-Val-Arg) within the NH2-terminal 16 kilodaltons of the molecule. S1 nuclease protection analysis confirmed that brain c-src RNA contains an 18-nucleotide insertion at the position of the extra six amino acids. This insertion occurs at a position that corresponds to a splice junction in the chicken and human c-src genes. The isolated c-src cDNA clone encodes a protein that displays an identical V8 peptide pattern to that observed in pp60c-src isolated from tissues of neuronal origin.

Full Text

Duke Authors

Cited Authors

  • Martinez, R; Mathey-Prevot, B; Bernards, A; Baltimore, D

Published Date

  • July 24, 1987

Published In

Volume / Issue

  • 237 / 4813

Start / End Page

  • 411 - 415

PubMed ID

  • 2440106

Pubmed Central ID

  • 2440106

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.2440106

Language

  • eng

Conference Location

  • United States