Specific transforming potential of oncogenes encoding protein-tyrosine kinases.
Several chimeric murine retroviruses were constructed to test whether the gag sequence of Abelson murine leukemia virus (A-MuLV) could influence the in vitro specificity of two sarcoma-inducing oncogenes: src of Rous sarcoma virus and fps of Fujinami sarcoma virus. Although the src- or fps- containing chimerae could transform fibroblasts, they were unable to mimic the action of A-MuLV in causing lymphoid transformation in vitro. A-MuLV-derived gag sequences could, however, functionally replace the 5' end of src and restore the transformation potential of a 5'-truncated src gene. To investigate this functional similarity, we replaced the gag sequence of an A-MuLV virus with the 5' end of src. This recombinant virus behaved like the A-MuLV virus from which it was derived: it transformed both fibroblasts and lymphoid cells in vitro. Taken together, these results suggest that lymphoid transformation in vitro is a specific property of abl and not of src or fps. Furthermore, it shows that a functional homology exists between the gag sequence of A-MuLV and the 5' end of src.
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Related Subject Headings
- Retroviridae
- Protein-Tyrosine Kinases
- Protein Kinases
- Plasmids
- Oncogenes
- Mice, Inbred Strains
- Mice
- Genes, Viral
- Genes
- Developmental Biology
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Retroviridae
- Protein-Tyrosine Kinases
- Protein Kinases
- Plasmids
- Oncogenes
- Mice, Inbred Strains
- Mice
- Genes, Viral
- Genes
- Developmental Biology