Specific transforming potential of oncogenes encoding protein-tyrosine kinases.

Journal Article

Several chimeric murine retroviruses were constructed to test whether the gag sequence of Abelson murine leukemia virus (A-MuLV) could influence the in vitro specificity of two sarcoma-inducing oncogenes: src of Rous sarcoma virus and fps of Fujinami sarcoma virus. Although the src- or fps- containing chimerae could transform fibroblasts, they were unable to mimic the action of A-MuLV in causing lymphoid transformation in vitro. A-MuLV-derived gag sequences could, however, functionally replace the 5' end of src and restore the transformation potential of a 5'-truncated src gene. To investigate this functional similarity, we replaced the gag sequence of an A-MuLV virus with the 5' end of src. This recombinant virus behaved like the A-MuLV virus from which it was derived: it transformed both fibroblasts and lymphoid cells in vitro. Taken together, these results suggest that lymphoid transformation in vitro is a specific property of abl and not of src or fps. Furthermore, it shows that a functional homology exists between the gag sequence of A-MuLV and the 5' end of src.

Full Text

Duke Authors

Cited Authors

  • Mathey-Prevot, B; Baltimore, D

Published Date

  • July 1, 1985

Published In

Volume / Issue

  • 4 / 7

Start / End Page

  • 1769 - 1774

PubMed ID

  • 2992940

International Standard Serial Number (ISSN)

  • 0261-4189

Language

  • eng

Conference Location

  • England