Preferential expression of the c-fps protein in chicken macrophages and granulocytic cells.

Journal Article (Journal Article)

We have studied the expression of the protein kinase activity of NCP98, the c-fps gene product, in several hemopoietic tissues of chickens as a function of the developmental stage of these organs. We found that in bone marrow, spleen, and bursa, maximum NCP98 kinase activity on a per-cell basis correlates with the peak of granulopoiesis in these organs. Furthermore, in a bovine serum albumin density gradient fractionation of bone marrow cells, granulocytic cells appeared to account for most of the NCP98 kinase activity. No correlation was found between the distribution of erythrocytic, lymphocytic, or thrombocytic cells and the distribution of the expression of NCP98 kinase activity. However, NCP98 protein and kinase activity were 10-fold higher in macrophages than in bone marrow. In addition, depletion by complement-mediated lysis of erythrocytic cells in bone marrow did not significantly reduce the total recovery of NCP98 kinase activity. These results argue for the specific expression of the c-fps gene product in granulocytic cells and macrophages.

Full Text

Duke Authors

Cited Authors

  • Samarut, J; Mathey-Prevot, B; Hanafusa, H

Published Date

  • May 1, 1985

Published In

Volume / Issue

  • 5 / 5

Start / End Page

  • 1067 - 1072

PubMed ID

  • 2987674

Pubmed Central ID

  • PMC366823

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/mcb.5.5.1067-1072.1985


  • eng

Conference Location

  • United States