Mutants of Fujinami sarcoma virus which are temperature sensitive for cellular transformation and protein kinase activity.

Published

Journal Article

Two temperature-sensitive mutants of Fujinami sarcoma virus were isolated and characterized. Cells infected with the mutants were temperature sensitive in focus formation, colony formation, increased sugar uptake, and synthesis of plasminogen activator. The changes between transformed and nontransformed states of cultures were completely reversible by shifting the temperature. A Fujinami sarcoma virus-specific protein of 130,000 daltons, p130, was synthesized in mutant-infected cells regardless of the temperature, but the immunoprecipitates of p130 from extracts of infected cells were active in protein kinase only when cells had been incubated at the permissive temperature. These results appear to indicate that p130 is the transforming protein of Fujinami sarcoma virus, and that its protein kinase activity plays a crucial role in cell transformation by this virus.

Full Text

Duke Authors

Cited Authors

  • Hanafusa, T; Mathey-Prevot, B; Feldman, RA; Hanafusa, H

Published Date

  • April 1, 1981

Published In

Volume / Issue

  • 38 / 1

Start / End Page

  • 347 - 355

PubMed ID

  • 6264108

Pubmed Central ID

  • 6264108

International Standard Serial Number (ISSN)

  • 0022-538X

Language

  • eng

Conference Location

  • United States