Guanylyl cyclase-D in the olfactory CO2 neurons is activated by bicarbonate.

Journal Article (Journal Article)

Atmospheric CO(2) is an important environmental cue that regulates several types of animal behavior. In mice, CO(2) responses of the olfactory sensory neurons (OSNs) require the activity of carbonic anhydrase to catalyze the conversion of CO(2) to bicarbonate and the opening of cGMP-sensitive ion channels. However, it remains unknown how the enhancement of bicarbonate levels results in cGMP production. Here, we show that bicarbonate activates cGMP-producing ability of guanylyl cyclase-D (GC-D), a membrane GC exclusively expressed in the CO(2)-responsive OSNs, by directly acting on the intracellular cyclase domain of GC-D. Also, the molecular mechanism for GC-D activation is distinct from the commonly believed model of "release from repression" for other membrane GCs. Our results contribute to our understanding of the molecular mechanisms of CO(2) sensing and suggest diverse mechanisms of molecular activation among membrane GCs.

Full Text

Duke Authors

Cited Authors

  • Sun, L; Wang, H; Hu, J; Han, J; Matsunami, H; Luo, M

Published Date

  • February 10, 2009

Published In

Volume / Issue

  • 106 / 6

Start / End Page

  • 2041 - 2046

PubMed ID

  • 19181845

Pubmed Central ID

  • PMC2644160

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

Digital Object Identifier (DOI)

  • 10.1073/pnas.0812220106


  • eng

Conference Location

  • United States