RTP family members induce functional expression of mammalian odorant receptors.

Published

Journal Article

Transport of G protein-coupled receptors (GPCRs) to the cell surface membrane is critical in order for the receptors to recognize their ligands. However, mammalian GPCR odorant receptors (ORs), when heterologously expressed in cells, are poorly expressed on the cell surface. Here we show that the transmembrane proteins RTP1 and RTP2 promote functional cell surface expression of ORs expressed in HEK293T cells. Genes encoding these proteins are expressed specifically in olfactory neurons. These proteins are associated with OR proteins and enhance the OR responses to odorants. Similar although weaker effects were seen with a third protein, REEP1. These findings suggest that RTP1 and RTP2 in particular play significant roles in the translocation of ORs to the plasma membrane as well as in the functioning of ORs. We have used this approach to identify active odorant ligands for ORs, providing a platform for screening the chemical selectivity of the large OR family.

Full Text

Duke Authors

Cited Authors

  • Saito, H; Kubota, M; Roberts, RW; Chi, Q; Matsunami, H

Published Date

  • November 2004

Published In

Volume / Issue

  • 119 / 5

Start / End Page

  • 679 - 691

PubMed ID

  • 15550249

Pubmed Central ID

  • 15550249

Electronic International Standard Serial Number (EISSN)

  • 1097-4172

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/j.cell.2004.11.021

Language

  • eng