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Cell binding specificity of mouse R-cadherin and chromosomal mapping of the gene.

Publication ,  Journal Article
Matsunami, H; Miyatani, S; Inoue, T; Copeland, NG; Gilbert, DJ; Jenkins, NA; Takeichi, M
Published in: J Cell Sci
September 1993

R-cadherin was originally identified as a chicken cadherin expressed by the retina. Here, we describe the identification of a mouse homologue of R-cadherin. We isolated mouse cDNAs encoding a cadherin with 94% identity in amino acid sequence to the chicken R-cadherin, and defined this molecule as mouse R-cadherin. L cells transfected with the mouse R-cadherin cDNA acquired a cadherin-mediated cell-cell adhesiveness as found for other cadherins. To examine the binding specificity of mouse R-cadherin, L cells expressing this cadherin (mRL) were mixed with L cells expressing chicken R-cadherin (cRL), mouse N-cadherin (mNL), mouse E-cadherin (mEL) and mouse P-cadherin (mPL). While mRL cells randomly intermixed with cRL cells, those cells aggregated separately from mEL or mPL cells. Mixing of mRL with mNL cells gave an intermediate result; that is, they formed both separate and chimeric aggregates, suggesting that R- and N-cadherin can interact with each other although each has a preference to bind to its own type. Similar properties were previously found for chicken R-cadherin. Thus, the cell binding specificity of R-cadherin is entirely conserved between the two species, suggesting a conserved role for this protein in morphogenesis. We also located the mouse R-cadherin gene to chromosome 2.

Duke Scholars

Published In

J Cell Sci

DOI

ISSN

0021-9533

Publication Date

September 1993

Volume

106 ( Pt 1)

Start / End Page

401 / 409

Location

England

Related Subject Headings

  • Species Specificity
  • Sequence Homology, Amino Acid
  • Sequence Alignment
  • Polymerase Chain Reaction
  • Muridae
  • Molecular Sequence Data
  • Mice, Inbred C57BL
  • Mice
  • L Cells
  • Genes
 

Citation

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MLA
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Matsunami, H., Miyatani, S., Inoue, T., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., & Takeichi, M. (1993). Cell binding specificity of mouse R-cadherin and chromosomal mapping of the gene. J Cell Sci, 106 ( Pt 1), 401–409. https://doi.org/10.1242/jcs.106.1.401
Matsunami, H., S. Miyatani, T. Inoue, N. G. Copeland, D. J. Gilbert, N. A. Jenkins, and M. Takeichi. “Cell binding specificity of mouse R-cadherin and chromosomal mapping of the gene.J Cell Sci 106 ( Pt 1) (September 1993): 401–9. https://doi.org/10.1242/jcs.106.1.401.
Matsunami H, Miyatani S, Inoue T, Copeland NG, Gilbert DJ, Jenkins NA, et al. Cell binding specificity of mouse R-cadherin and chromosomal mapping of the gene. J Cell Sci. 1993 Sep;106 ( Pt 1):401–9.
Matsunami, H., et al. “Cell binding specificity of mouse R-cadherin and chromosomal mapping of the gene.J Cell Sci, vol. 106 ( Pt 1), Sept. 1993, pp. 401–09. Pubmed, doi:10.1242/jcs.106.1.401.
Matsunami H, Miyatani S, Inoue T, Copeland NG, Gilbert DJ, Jenkins NA, Takeichi M. Cell binding specificity of mouse R-cadherin and chromosomal mapping of the gene. J Cell Sci. 1993 Sep;106 ( Pt 1):401–409.
Journal cover image

Published In

J Cell Sci

DOI

ISSN

0021-9533

Publication Date

September 1993

Volume

106 ( Pt 1)

Start / End Page

401 / 409

Location

England

Related Subject Headings

  • Species Specificity
  • Sequence Homology, Amino Acid
  • Sequence Alignment
  • Polymerase Chain Reaction
  • Muridae
  • Molecular Sequence Data
  • Mice, Inbred C57BL
  • Mice
  • L Cells
  • Genes