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Chlamydia protease-like activity factor (CPAF): characterization of proteolysis activity in vitro and development of a nanomolar affinity CPAF zymogen-derived inhibitor.

Publication ,  Journal Article
Bednar, MM; Jorgensen, I; Valdivia, RH; McCafferty, DG
Published in: Biochemistry
September 6, 2011

During infection of epithelial cells, the obligate intracellular pathogen Chlamydia trachomatis secretes the serine protease Chlamydia protease-like activity factor (CPAF) into the host cytosol to regulate a range of host cellular processes through targeted proteolysis. Here we report the development of an in vitro assay for the enzyme and the discovery of a cell-permeable CPAF zymogen-based peptide inhibitor with nanomolar inhibitory affinity. Treating C. trachomatis-infected HeLa cells with this inhibitor prevented CPAF cleavage of the intermediate filament vimentin and led to the loss of vimentin cage surrounding the intracellular vacuole. Because Chlamydia is a genetically intractable organism, this inhibitor may serve as a tool for understanding the role of CPAF in pathogenesis.

Duke Scholars

Published In

Biochemistry

DOI

EISSN

1520-4995

Publication Date

September 6, 2011

Volume

50

Issue

35

Start / End Page

7441 / 7443

Location

United States

Related Subject Headings

  • Vimentin
  • Vacuoles
  • Protein Binding
  • Protease Inhibitors
  • Peptides
  • Peptide Hydrolases
  • Molecular Sequence Data
  • Intracellular Fluid
  • Humans
  • Hela Cells
 

Citation

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Bednar, M. M., Jorgensen, I., Valdivia, R. H., & McCafferty, D. G. (2011). Chlamydia protease-like activity factor (CPAF): characterization of proteolysis activity in vitro and development of a nanomolar affinity CPAF zymogen-derived inhibitor. Biochemistry, 50(35), 7441–7443. https://doi.org/10.1021/bi201098r
Bednar, Maria M., Ine Jorgensen, Raphael H. Valdivia, and Dewey G. McCafferty. “Chlamydia protease-like activity factor (CPAF): characterization of proteolysis activity in vitro and development of a nanomolar affinity CPAF zymogen-derived inhibitor.Biochemistry 50, no. 35 (September 6, 2011): 7441–43. https://doi.org/10.1021/bi201098r.
Bednar, Maria M., et al. “Chlamydia protease-like activity factor (CPAF): characterization of proteolysis activity in vitro and development of a nanomolar affinity CPAF zymogen-derived inhibitor.Biochemistry, vol. 50, no. 35, Sept. 2011, pp. 7441–43. Pubmed, doi:10.1021/bi201098r.
Journal cover image

Published In

Biochemistry

DOI

EISSN

1520-4995

Publication Date

September 6, 2011

Volume

50

Issue

35

Start / End Page

7441 / 7443

Location

United States

Related Subject Headings

  • Vimentin
  • Vacuoles
  • Protein Binding
  • Protease Inhibitors
  • Peptides
  • Peptide Hydrolases
  • Molecular Sequence Data
  • Intracellular Fluid
  • Humans
  • Hela Cells