Generation of ramoplanin-resistant Staphylococcus aureus.
Journal Article (Journal Article)
Ramoplanin is a lipoglycodepsipeptide antimicrobial active against clinically important Gram-positive bacteria including methicillin-resistant Staphylococcus aureus. To proactively examine ramoplanin resistance, we subjected S. aureus NCTC 8325-4 to serial passage in the presence of increasing concentrations of ramoplanin, generating the markedly resistant strain RRSA16. Susceptibility testing of RRSA16 revealed the unanticipated acquisition of cross-resistance to vancomycin and nisin. RRSA16 displayed phenotypes, including a thickened cell wall and reduced susceptibility to Triton X-100-induced autolysis, which are associated with vancomycin intermediate-resistant S. aureus strains. Passage of RRSA16 for 18 days in a drug-free medium yielded strain R16-18d with restored antibiotic susceptibility. The RRSA16 isolate may be used to identify the genetic and biochemical basis for ramoplanin resistance and to further our understanding of the evolution of antibiotic cross-resistance mechanisms in S. aureus.
Full Text
Duke Authors
Cited Authors
- Schmidt, JW; Greenough, A; Burns, M; Luteran, AE; McCafferty, DG
Published Date
- September 2010
Published In
Volume / Issue
- 310 / 2
Start / End Page
- 104 - 111
PubMed ID
- 20659164
Pubmed Central ID
- PMC3596103
Electronic International Standard Serial Number (EISSN)
- 1574-6968
International Standard Serial Number (ISSN)
- 0378-1097
Digital Object Identifier (DOI)
- 10.1111/j.1574-6968.2010.02051.x
Language
- eng