Generation of ramoplanin-resistant Staphylococcus aureus.

Published

Journal Article

Ramoplanin is a lipoglycodepsipeptide antimicrobial active against clinically important Gram-positive bacteria including methicillin-resistant Staphylococcus aureus. To proactively examine ramoplanin resistance, we subjected S. aureus NCTC 8325-4 to serial passage in the presence of increasing concentrations of ramoplanin, generating the markedly resistant strain RRSA16. Susceptibility testing of RRSA16 revealed the unanticipated acquisition of cross-resistance to vancomycin and nisin. RRSA16 displayed phenotypes, including a thickened cell wall and reduced susceptibility to Triton X-100-induced autolysis, which are associated with vancomycin intermediate-resistant S. aureus strains. Passage of RRSA16 for 18 days in a drug-free medium yielded strain R16-18d with restored antibiotic susceptibility. The RRSA16 isolate may be used to identify the genetic and biochemical basis for ramoplanin resistance and to further our understanding of the evolution of antibiotic cross-resistance mechanisms in S. aureus.

Full Text

Duke Authors

Cited Authors

  • Schmidt, JW; Greenough, A; Burns, M; Luteran, AE; McCafferty, DG

Published Date

  • September 2010

Published In

Volume / Issue

  • 310 / 2

Start / End Page

  • 104 - 111

PubMed ID

  • 20659164

Pubmed Central ID

  • 20659164

Electronic International Standard Serial Number (EISSN)

  • 1574-6968

International Standard Serial Number (ISSN)

  • 0378-1097

Digital Object Identifier (DOI)

  • 10.1111/j.1574-6968.2010.02051.x

Language

  • eng