Incorporation into lipids of labelled acetate, oleate, and phosphate by canine arteries, veins, and vein grafts
The incorporation into lipids of (14C) labeled acetate, (3H) labeled oleate and (32P) labeled phosphate by canine vascular tissue was studied. Jugular vein interposition grafts were placed into the carotid arteries of 20 dogs. Eight dogs were maintained on an atherogenic regimen. Grafts remained in situ for an average of 14 months. Incorporation of precursors into lipids was determined in vitro in an organ culture system over 24 hours. Vein grafts from normal diet animals incorporated acetate and oleate into lipids more actively than artery or vein. The distribution of lipid radioactivity was similar to undisturbed vein. Incorporation into cholesteryl ester constituted a small fraction of the total but was significantly increased in normal diet vein grafts. This change was accentuated by long term exposure to hypercholesterolemia. Vein graft tissue in this model may be more susceptible than the artery it is replacing to potentially injurious biochemical alterations such as cholesteryl ester accumulation.