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Side-chain specificities and molecular modelling of peptide determinants for two anti-Sm B/B' autoantibodies.

Publication ,  Journal Article
James, JA; McClain, MT; Koelsch, G; Williams, DG; Harley, JB
Published in: J Autoimmun
February 1999

Autoantibodies binding the Sm B and B' peptides (B/B') are commonly associated with systemic lupus erythematosus in man and in MRL lpr/lpr mice. The linear antigenic regions of two anti-Sm B/B' murine monoclonal auto-antibodies have been mapped using overlapping octapeptides. Unique epitopes are identified by each antibody. Monoclonal KSm-5 recognizes the peptide, PPPGMRPP, which is repeated three times in the Sm B polypeptide. KSm 3 preferably binds to two similar, almost neighboring octapeptides, PPPGIRGP and PGIRGPPP. The two monoclonal antibodies do not cross react. These regions of Sm B/B' are major areas of antigenicity in human sera. Amino acid deletion and substitution in antigenic octapeptides show that binding to the KSm-5 epitope is lost with most modifications. Molecular dynamic modelling suggests that when PPPGMRPP is substituted in the sixth position arginine, KSm/5 binding may be associated with a shared peptide backbone structure rather than charge or hydrophobicity of the substituted amino acid. In contrast, binding of KSm-3 to PPPGIRGP is abolished when the sixth position arginine is substituted by any other amino acid. Substitution at arginine and modelling experiments, therefore, suggest very different mech-anisms of binding. Autoantibodies may bind quite different features of similar peptide structures.

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Published In

J Autoimmun

DOI

ISSN

0896-8411

Publication Date

February 1999

Volume

12

Issue

1

Start / End Page

43 / 49

Location

England

Related Subject Headings

  • snRNP Core Proteins
  • Ribonucleoproteins, Small Nuclear
  • Protein Conformation
  • Oligopeptides
  • Molecular Sequence Data
  • Models, Molecular
  • Mice
  • Immunology
  • Epitopes
  • Computer Simulation
 

Citation

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James, J. A., McClain, M. T., Koelsch, G., Williams, D. G., & Harley, J. B. (1999). Side-chain specificities and molecular modelling of peptide determinants for two anti-Sm B/B' autoantibodies. J Autoimmun, 12(1), 43–49. https://doi.org/10.1006/jaut.1998.0252
James, J. A., M. T. McClain, G. Koelsch, D. G. Williams, and J. B. Harley. “Side-chain specificities and molecular modelling of peptide determinants for two anti-Sm B/B' autoantibodies.J Autoimmun 12, no. 1 (February 1999): 43–49. https://doi.org/10.1006/jaut.1998.0252.
James JA, McClain MT, Koelsch G, Williams DG, Harley JB. Side-chain specificities and molecular modelling of peptide determinants for two anti-Sm B/B' autoantibodies. J Autoimmun. 1999 Feb;12(1):43–9.
James, J. A., et al. “Side-chain specificities and molecular modelling of peptide determinants for two anti-Sm B/B' autoantibodies.J Autoimmun, vol. 12, no. 1, Feb. 1999, pp. 43–49. Pubmed, doi:10.1006/jaut.1998.0252.
James JA, McClain MT, Koelsch G, Williams DG, Harley JB. Side-chain specificities and molecular modelling of peptide determinants for two anti-Sm B/B' autoantibodies. J Autoimmun. 1999 Feb;12(1):43–49.
Journal cover image

Published In

J Autoimmun

DOI

ISSN

0896-8411

Publication Date

February 1999

Volume

12

Issue

1

Start / End Page

43 / 49

Location

England

Related Subject Headings

  • snRNP Core Proteins
  • Ribonucleoproteins, Small Nuclear
  • Protein Conformation
  • Oligopeptides
  • Molecular Sequence Data
  • Models, Molecular
  • Mice
  • Immunology
  • Epitopes
  • Computer Simulation