Quantitative measurement of cell adhesion using centrifugal force.
Publication
, Journal Article
McClay, DR; Hertzler, PL
Published in: Current protocols in cell biology
May 2001
In order to study the biophysical forces involved in cell-substrate (or cell-cell) adhesion, it is necessary to measure the strength of adhesion. Two questions can be addressed using the centrifugal cell adhesion assay provided in this unit: what is the ligand-receptor affinity for cells adhering at 4 degrees C and what is the strength of the ATP-dependent processes that strengthen adhesion at 37 degrees C. In both cases, the strength of adhesion is measured as the resistance to disruption in the presence of a measured centrifugal force.
Duke Scholars
Published In
Current protocols in cell biology
DOI
EISSN
1934-2616
ISSN
1934-2500
Publication Date
May 2001
Volume
Chapter 9
Start / End Page
Unit / 9.2
Related Subject Headings
- Temperature
- Ligands
- Centrifugation
- Cell Adhesion Molecules
- Cell Adhesion
- Adenosine Triphosphate
- 3101 Biochemistry and cell biology
Citation
APA
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ICMJE
MLA
NLM
McClay, D. R., & Hertzler, P. L. (2001). Quantitative measurement of cell adhesion using centrifugal force. Current Protocols in Cell Biology, Chapter 9, Unit-9.2. https://doi.org/10.1002/0471143030.cb0902s00
McClay, D. R., and P. L. Hertzler. “Quantitative measurement of cell adhesion using centrifugal force.” Current Protocols in Cell Biology Chapter 9 (May 2001): Unit-9.2. https://doi.org/10.1002/0471143030.cb0902s00.
McClay DR, Hertzler PL. Quantitative measurement of cell adhesion using centrifugal force. Current protocols in cell biology. 2001 May;Chapter 9:Unit-9.2.
McClay, D. R., and P. L. Hertzler. “Quantitative measurement of cell adhesion using centrifugal force.” Current Protocols in Cell Biology, vol. Chapter 9, May 2001, p. Unit-9.2. Epmc, doi:10.1002/0471143030.cb0902s00.
McClay DR, Hertzler PL. Quantitative measurement of cell adhesion using centrifugal force. Current protocols in cell biology. 2001 May;Chapter 9:Unit-9.2.
Published In
Current protocols in cell biology
DOI
EISSN
1934-2616
ISSN
1934-2500
Publication Date
May 2001
Volume
Chapter 9
Start / End Page
Unit / 9.2
Related Subject Headings
- Temperature
- Ligands
- Centrifugation
- Cell Adhesion Molecules
- Cell Adhesion
- Adenosine Triphosphate
- 3101 Biochemistry and cell biology