Quantitative measurement of cell adhesion using centrifugal force.

Journal Article

In order to study the biophysical forces involved in cell-substrate (or cell-cell) adhesion, it is necessary to measure the strength of adhesion. Two questions can be addressed using the centrifugal cell adhesion assay provided in this unit: what is the ligand-receptor affinity for cells adhering at 4 degrees C and what is the strength of the ATP-dependent processes that strengthen adhesion at 37 degrees C. In both cases, the strength of adhesion is measured as the resistance to disruption in the presence of a measured centrifugal force.

Full Text

Duke Authors

Cited Authors

  • McClay, DR; Hertzler, PL

Published Date

  • May 2001

Published In

Volume / Issue

  • Chapter 9 /

Start / End Page

  • Unit - 9.2

PubMed ID

  • 18228392

Electronic International Standard Serial Number (EISSN)

  • 1934-2616

Digital Object Identifier (DOI)

  • 10.1002/0471143030.cb0902s00

Language

  • eng

Conference Location

  • United States