Neuronal-glial interactions: complexity of neurite outgrowth correlates with substrate adhesivity of serotonergic neurons.

Journal Article (Journal Article)

To study the interactions between neurons of known transmitter phenotype and non-neuronal cells of glial or fibroblastic origin, serotonergic (5-HT) neurons were tested for their strength of adhesion and neurite outgrowth patterns on substrates of astrocytes or fibroblasts using a cell adhesion assay for transmitter-identified neurons, and morphometry of immunocytochemically stained neurons in dissociated cell cultures. Both the strength of adhesion and the rate and complexity of neurite outgrowth by 5-HT neurons were significantly greater on substrates of astrocytes compared to fibroblasts. These results provide evidence that 5-HT neurons can interact selectively with glia via cell surface determinants, and that this process may be important for the development of complex (dendrite-like) neuritic arbors. The methods developed in this study will be useful for future studies of interactions between transmitter-identified neurons and glial cells during ontogeny of the embryonic brain.

Full Text

Duke Authors

Cited Authors

  • Lieth, E; McClay, DR; Lauder, JM

Published Date

  • January 1990

Published In

Volume / Issue

  • 3 / 3

Start / End Page

  • 169 - 179

PubMed ID

  • 2141593

Electronic International Standard Serial Number (EISSN)

  • 1098-1136

International Standard Serial Number (ISSN)

  • 0894-1491

Digital Object Identifier (DOI)

  • 10.1002/glia.440030304


  • eng