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Direct interactions between corepressors and coactivators permit the integration of nuclear receptor-mediated repression and activation.

Publication ,  Journal Article
Li, X; Kimbrel, EA; Kenan, DJ; McDonnell, DP
Published in: Mol Endocrinol
July 2002

The unliganded thyroid hormone receptor beta (TRbeta) represses the basal transcriptional activity of target genes, in part through interactions with the nuclear receptor corepressor (N-CoR). In this study we have identified a rather unexpected interaction between N-CoR and the nuclear receptor coactivator ACTR. We have demonstrated in vitro and in intact cells that N-CoR directly associates with ACTR and that the interaction surfaces on N-CoR and ACTR are distinct from those required for TR binding. The significance of this finding was demonstrated by showing that N-CoR facilitates an interaction between unliganded-TRbeta and ACTR. One possible consequence of the formation of the trimeric complex of N-CoR/ACTR/unliganded-TR is that N-CoR may raise the local concentration of ACTR at target gene promoters. In support of this hypothesis it was demonstrated that the presence of N-CoR can enhance TRbeta-mediated transcriptional activation. It is proposed, therefore, that TRbeta- mediated activation and repression are integrally linked in a manner that is not predicted by the current models of nuclear receptor action.

Duke Scholars

Published In

Mol Endocrinol

DOI

ISSN

0888-8809

Publication Date

July 2002

Volume

16

Issue

7

Start / End Page

1482 / 1491

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Thyroid Hormone Receptors beta
  • Saccharomyces cerevisiae Proteins
  • Repressor Proteins
  • Recombinant Proteins
  • Receptors, Thyroid Hormone
  • Promoter Regions, Genetic
  • Nuclear Receptor Coactivator 2
  • Nuclear Receptor Coactivator 1
 

Citation

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MLA
NLM
Li, X., Kimbrel, E. A., Kenan, D. J., & McDonnell, D. P. (2002). Direct interactions between corepressors and coactivators permit the integration of nuclear receptor-mediated repression and activation. Mol Endocrinol, 16(7), 1482–1491. https://doi.org/10.1210/mend.16.7.0860
Li, Xiaolin, Erin A. Kimbrel, Daniel J. Kenan, and Donald P. McDonnell. “Direct interactions between corepressors and coactivators permit the integration of nuclear receptor-mediated repression and activation.Mol Endocrinol 16, no. 7 (July 2002): 1482–91. https://doi.org/10.1210/mend.16.7.0860.
Li, Xiaolin, et al. “Direct interactions between corepressors and coactivators permit the integration of nuclear receptor-mediated repression and activation.Mol Endocrinol, vol. 16, no. 7, July 2002, pp. 1482–91. Pubmed, doi:10.1210/mend.16.7.0860.

Published In

Mol Endocrinol

DOI

ISSN

0888-8809

Publication Date

July 2002

Volume

16

Issue

7

Start / End Page

1482 / 1491

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Thyroid Hormone Receptors beta
  • Saccharomyces cerevisiae Proteins
  • Repressor Proteins
  • Recombinant Proteins
  • Receptors, Thyroid Hormone
  • Promoter Regions, Genetic
  • Nuclear Receptor Coactivator 2
  • Nuclear Receptor Coactivator 1