Expression of recombinant human methylmalonyl-CoA mutase: in primary mut fibroblasts and Saccharomyces cerevisiae.

Journal Article (Journal Article)

Methylmalonyl-CoA mutase is an adenosylcobalamin-dependent enzyme which catalyzes isomerization of methylmalonyl-CoA to succinyl-CoA. Previous reports have described cloning and sequencing of a cDNA for human methylmalonyl-CoA mutase. This clone does not express an active apoenzyme after gene transfer into primary MCM-deficient fibroblasts and contains several sequences which differ from the consensus sequence of other cDNA clones. We describe reconstruction of a functional MCM cDNA and expression of recombinant enzyme activity in primary fibroblasts and Saccharomyces cerevisiae. This consensus human MCM cDNA is capable of complementing the inherited defect in mut MMA and overexpressing an enzyme in yeast with kinetic properties indistinguishable from the enzyme in murine or human tissues.

Full Text

Duke Authors

Cited Authors

  • Andrews, E; Jansen, R; Crane, AM; Cholin, S; McDonnell, D; Ledley, FD

Published Date

  • October 1993

Published In

Volume / Issue

  • 50 / 2

Start / End Page

  • 135 - 144

PubMed ID

  • 7903149

International Standard Serial Number (ISSN)

  • 0885-4505

Digital Object Identifier (DOI)

  • 10.1006/bmmb.1993.1055


  • eng

Conference Location

  • United States