Expression of recombinant human methylmalonyl-CoA mutase: in primary mut fibroblasts and Saccharomyces cerevisiae.
Journal Article (Journal Article)
Methylmalonyl-CoA mutase is an adenosylcobalamin-dependent enzyme which catalyzes isomerization of methylmalonyl-CoA to succinyl-CoA. Previous reports have described cloning and sequencing of a cDNA for human methylmalonyl-CoA mutase. This clone does not express an active apoenzyme after gene transfer into primary MCM-deficient fibroblasts and contains several sequences which differ from the consensus sequence of other cDNA clones. We describe reconstruction of a functional MCM cDNA and expression of recombinant enzyme activity in primary fibroblasts and Saccharomyces cerevisiae. This consensus human MCM cDNA is capable of complementing the inherited defect in mut MMA and overexpressing an enzyme in yeast with kinetic properties indistinguishable from the enzyme in murine or human tissues.
Full Text
Duke Authors
Cited Authors
- Andrews, E; Jansen, R; Crane, AM; Cholin, S; McDonnell, D; Ledley, FD
Published Date
- October 1993
Published In
Volume / Issue
- 50 / 2
Start / End Page
- 135 - 144
PubMed ID
- 7903149
International Standard Serial Number (ISSN)
- 0885-4505
Digital Object Identifier (DOI)
- 10.1006/bmmb.1993.1055
Language
- eng
Conference Location
- United States