Identification of novel steroid-response elements.

Published

Journal Article

A rapid method for defining novel steroid-responsive elements has been developed. Large libraries of degenerate oligonucleotides were analyzed using a yeast-based screen to identify estrogen-responsive DNA sequences. From a library of 40,000 recombinants, seven estrogen-responsive clones were identified. When sequenced, these elements showed remarkable diversity and were different from the consensus vitellogenin A2 ERE. One surprising result was the presence of the two half sites as direct repeats in some of the clones. This implies that in vivo estrogen receptor can bind and transactivate yeast genes through response elements in which the two half sites align as direct repeats. This protocol requires no purified protein and specifically selects for functional response elements. It has a wide application in the study of any transcription factor/DNA interaction.

Full Text

Duke Authors

Cited Authors

  • Nawaz, Z; Tsai, MJ; McDonnell, DP; O'Malley, BW

Published Date

  • 1992

Published In

Volume / Issue

  • 2 / 1

Start / End Page

  • 39 - 47

PubMed ID

  • 1617301

Pubmed Central ID

  • 1617301

International Standard Serial Number (ISSN)

  • 1052-2166

Language

  • eng

Conference Location

  • United States