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Identification of novel steroid-response elements.

Publication ,  Journal Article
Nawaz, Z; Tsai, MJ; McDonnell, DP; O'Malley, BW
Published in: Gene Expr
1992
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A rapid method for defining novel steroid-responsive elements has been developed. Large libraries of degenerate oligonucleotides were analyzed using a yeast-based screen to identify estrogen-responsive DNA sequences. From a library of 40,000 recombinants, seven estrogen-responsive clones were identified. When sequenced, these elements showed remarkable diversity and were different from the consensus vitellogenin A2 ERE. One surprising result was the presence of the two half sites as direct repeats in some of the clones. This implies that in vivo estrogen receptor can bind and transactivate yeast genes through response elements in which the two half sites align as direct repeats. This protocol requires no purified protein and specifically selects for functional response elements. It has a wide application in the study of any transcription factor/DNA interaction.

Duke Scholars

Donald Patrick McDonnell
Author Donald Patrick McDonnell Pharmacology & Cancer Biology

Published In

Gene Expr

ISSN

1052-2166

Publication Date

1992

Volume

2

Issue

1

Start / End Page

39 / 47

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Trans-Activators
  • Sequence Homology, Nucleic Acid
  • Saccharomyces cerevisiae
  • Receptors, Steroid
  • Receptors, Estrogen
  • Molecular Sequence Data
  • Humans
  • Enhancer Elements, Genetic
  • Databases, Factual
 

Citation

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Nawaz, Z., Tsai, M. J., McDonnell, D. P., & O’Malley, B. W. (1992). Identification of novel steroid-response elements. Gene Expr, 2(1), 39–47.
Nawaz, Z., M. J. Tsai, D. P. McDonnell, and B. W. O’Malley. “Identification of novel steroid-response elements.” Gene Expr 2, no. 1 (1992): 39–47.
Nawaz Z, Tsai MJ, McDonnell DP, O’Malley BW. Identification of novel steroid-response elements. Gene Expr. 1992;2(1):39–47.
Nawaz, Z., et al. “Identification of novel steroid-response elements.” Gene Expr, vol. 2, no. 1, 1992, pp. 39–47.
Nawaz Z, Tsai MJ, McDonnell DP, O’Malley BW. Identification of novel steroid-response elements. Gene Expr. 1992;2(1):39–47.
Journal cover image

Published In

Gene Expr

ISSN

1052-2166

Publication Date

1992

Volume

2

Issue

1

Start / End Page

39 / 47

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Trans-Activators
  • Sequence Homology, Nucleic Acid
  • Saccharomyces cerevisiae
  • Receptors, Steroid
  • Receptors, Estrogen
  • Molecular Sequence Data
  • Humans
  • Enhancer Elements, Genetic
  • Databases, Factual