Reconstitution of the vitamin D-responsive osteocalcin transcription unit in Saccharomyces cerevisiae.

Published

Journal Article

The human osteocalcin gene is regulated in mammalian osteoblasts by 1,25(OH)2D3-dependent and -independent mechanisms. The sequences responsible for this activity have been mapped to within the -1339 region of the gene. We show here that this enhancer region functions analogously in Saccharomyces cerevisiae cells engineered to produce active 1,25(OH)2D3 receptor. When fused to the proximal promoter elements of the yeast iso-1-cytochrome c gene, the enhancer demonstrated substantial promoter activity. This activity was elevated further by 1,25(OH)2D3 when the reporter constructs were assayed in cells containing the 1,25(OH)2D3 receptor. This system affords a model for 1,25(OH)2D3 action and represents a simple assay system that will enable definition of the important cis-acting regulatory sequences within the osteocalcin gene and identification of their cognate transcription factors.

Full Text

Duke Authors

Cited Authors

  • McDonnell, DP; Pike, JW; Drutz, DJ; Butt, TR; O'Malley, BW

Published Date

  • August 1, 1989

Published In

Volume / Issue

  • 9 / 8

Start / End Page

  • 3517 - 3523

PubMed ID

  • 2552296

Pubmed Central ID

  • 2552296

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/mcb.9.8.3517

Language

  • eng

Conference Location

  • United States