The effect of charge-reversal amphiphile spacer composition on DNA and siRNA delivery.

Journal Article (Journal Article)

A series of charge-reversal amphiphiles with different spacers separating the headgroup from the hydrophobic chains are described for delivery of DNA and siRNA. Among them, the amphiphiles possessing a glycine spacer (e.g., B-GlyGly) showed effective DNA transfection in CHO and NIH 3T3 cells, as well as siRNA gene knockdown in HepG2 and UASMC cells. Ethidium bromide quenching assays revealed that DNA was released the fastest from the lipoplex of B-GlyGly in the presence of esterase. Also, X-ray diffraction results indicated that the DNA was located between the adjacent lipid bilayers in the lipoplex of B-GlyGly. These distinct features appear to be required for high transfection activity.

Full Text

Duke Authors

Cited Authors

  • Zhang, X-X; Prata, CAH; McIntosh, TJ; Barthélémy, P; Grinstaff, MW

Published Date

  • May 19, 2010

Published In

Volume / Issue

  • 21 / 5

Start / End Page

  • 988 - 993

PubMed ID

  • 20433165

Pubmed Central ID

  • PMC3052776

Electronic International Standard Serial Number (EISSN)

  • 1520-4812

Digital Object Identifier (DOI)

  • 10.1021/bc9005464

Language

  • eng

Conference Location

  • United States