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X-ray diffraction to determine the thickness of raft and nonraft bilayers.

Publication ,  Journal Article
McIntosh, TJ
Published in: Methods Mol Biol
2007

Low-angle X-ray diffraction is a powerful method to analyze the structure of membrane bilayers. Specifically, the technique can be used to determine accurately the thickness of fully hydrated bilayers. Herein details are presented showing how this technique can measure the difference in thickness of bilayers in detergent-resistant membranes and detergent-soluble membranes extracted from model systems known to contain both raft and nonraft domains. The observed thickness difference may be critical in the sorting of transmembrane proteins between raft and nonraft bilayers.

Duke Scholars

Published In

Methods Mol Biol

DOI

ISSN

1064-3745

Publication Date

2007

Volume

398

Start / End Page

221 / 230

Location

United States

Related Subject Headings

  • X-Ray Diffraction
  • Sphingomyelins
  • Solubility
  • Phosphatidylcholines
  • Membrane Proteins
  • Membrane Microdomains
  • Lipid Bilayers
  • Hydrocarbons
  • Electrons
  • Developmental Biology
 

Citation

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ICMJE
MLA
NLM
McIntosh, T. J. (2007). X-ray diffraction to determine the thickness of raft and nonraft bilayers. Methods Mol Biol, 398, 221–230. https://doi.org/10.1007/978-1-59745-513-8_15
McIntosh, Thomas J. “X-ray diffraction to determine the thickness of raft and nonraft bilayers.Methods Mol Biol 398 (2007): 221–30. https://doi.org/10.1007/978-1-59745-513-8_15.
McIntosh TJ. X-ray diffraction to determine the thickness of raft and nonraft bilayers. Methods Mol Biol. 2007;398:221–30.
McIntosh, Thomas J. “X-ray diffraction to determine the thickness of raft and nonraft bilayers.Methods Mol Biol, vol. 398, 2007, pp. 221–30. Pubmed, doi:10.1007/978-1-59745-513-8_15.
McIntosh TJ. X-ray diffraction to determine the thickness of raft and nonraft bilayers. Methods Mol Biol. 2007;398:221–230.

Published In

Methods Mol Biol

DOI

ISSN

1064-3745

Publication Date

2007

Volume

398

Start / End Page

221 / 230

Location

United States

Related Subject Headings

  • X-Ray Diffraction
  • Sphingomyelins
  • Solubility
  • Phosphatidylcholines
  • Membrane Proteins
  • Membrane Microdomains
  • Lipid Bilayers
  • Hydrocarbons
  • Electrons
  • Developmental Biology