Charge-reversal amphiphiles for gene delivery.

Journal Article (Journal Article)

Delivering a missing gene or a functional substitute of a defective gene has the potential to revolutionize current medical care. Of the two gene delivery approaches, viral and synthetic vectors, synthetic cationic vectors possess several practical advantages but suffer from poor transfection efficiency. A new approach to gene delivery using charge-reversal amphiphiles is described. This synthetic vector transforms from a cationic to an anionic amphiphile intracellularly. This amphiphile performs two roles: first, it binds and then releases DNA, and second, as an anionic multicharged amphiphile, it destabilizes lipid bilayers. A charge-reversal amphiphile was synthesized and fully characterized, including the supramolecular complex it forms with DNA. Enhanced gene transfection was observed using these vectors compared to current cationic amphiphiles.

Full Text

Duke Authors

Cited Authors

  • Prata, CAH; Zhao, Y; Barthelemy, P; Li, Y; Luo, D; McIntosh, TJ; Lee, SJ; Grinstaff, MW

Published Date

  • October 6, 2004

Published In

Volume / Issue

  • 126 / 39

Start / End Page

  • 12196 - 12197

PubMed ID

  • 15453715

International Standard Serial Number (ISSN)

  • 0002-7863

Digital Object Identifier (DOI)

  • 10.1021/ja0474906


  • eng

Conference Location

  • United States