Reduction of TrkB expression de novo in the adult mouse impairs epileptogenesis in the kindling model.

Published

Journal Article

Elucidating the mechanisms of epileptogenesis in molecular terms can identify targets for therapies aimed at preventing epileptogenesis or limiting its progression. Genetic perturbations have implicated signaling by the neurotrophin, BDNF, and its receptor, TrkB, in limbic epileptogenesis. Whether this signaling is critical to epileptogenesis in the adult brain is unclear. We sought to determine whether reduced expression of TrkB de novo in the mature brain is sufficient to impair epileptogenesis in the kindling model. Treatment of adult Act-CreER TrkB(flox/flox) mice with tamoxifen resulted in modest reductions of TrkB protein expression de novo in the adult that were detected in hippocampus but not other brain regions. Modest reduction of hippocampal TrkB content inhibited epileptogenesis induced by stimulation of hippocampus or amygdala. The data support the conclusion that reduction of TrkB expression in hippocampus de novo in the mature brain impairs epileptogenesis in the kindling model. These findings advance TrkB and its downstream signaling pathways as attractive targets for limiting the progression of epileptogenesis.

Full Text

Duke Authors

Cited Authors

  • Kotloski, R; McNamara, JO

Published Date

  • June 2010

Published In

Volume / Issue

  • 20 / 6

Start / End Page

  • 713 - 723

PubMed ID

  • 19603519

Pubmed Central ID

  • 19603519

Electronic International Standard Serial Number (EISSN)

  • 1098-1063

Digital Object Identifier (DOI)

  • 10.1002/hipo.20673

Language

  • eng

Conference Location

  • United States