Remote control of neuronal activity in transgenic mice expressing evolved G protein-coupled receptors.

Published

Journal Article

Examining the behavioral consequences of selective CNS neuronal activation is a powerful tool for elucidating mammalian brain function in health and disease. Newly developed genetic, pharmacological, and optical tools allow activation of neurons with exquisite spatiotemporal resolution; however, the inaccessibility to light of widely distributed neuronal populations and the invasiveness required for activation by light or infused ligands limit the utility of these methods. To overcome these barriers, we created transgenic mice expressing an evolved G protein-coupled receptor (hM3Dq) selectively activated by the pharmacologically inert, orally bioavailable drug clozapine-N-oxide (CNO). Here, we expressed hM3Dq in forebrain principal neurons. Local field potential and single-neuron recordings revealed that peripheral administration of CNO activated hippocampal neurons selectively in hM3Dq-expressing mice. Behavioral correlates of neuronal activation included increased locomotion, stereotypy, and limbic seizures. These results demonstrate a powerful chemical-genetic tool for remotely controlling the activity of discrete populations of neurons in vivo.

Full Text

Duke Authors

Cited Authors

  • Alexander, GM; Rogan, SC; Abbas, AI; Armbruster, BN; Pei, Y; Allen, JA; Nonneman, RJ; Hartmann, J; Moy, SS; Nicolelis, MA; McNamara, JO; Roth, BL

Published Date

  • July 16, 2009

Published In

Volume / Issue

  • 63 / 1

Start / End Page

  • 27 - 39

PubMed ID

  • 19607790

Pubmed Central ID

  • 19607790

Electronic International Standard Serial Number (EISSN)

  • 1097-4199

Digital Object Identifier (DOI)

  • 10.1016/j.neuron.2009.06.014

Language

  • eng

Conference Location

  • United States