Measurement of NMDA receptor protein subunits in discrete hippocampal regions of kindled animals.
Published
Journal Article
Kindling refers to a phenomenon in which repeated application of initially subconvulsive electrical stimulations produces limbic and clonic motor seizures of progressively increasing severity. Once established, the increased excitability is lifelong. A diversity of studies demonstrate that kindling results in long lasting (28 days) alterations of the functional and pharmacologic properties of NMDA receptors, indicating that kindling may cause changes intrinsic to the NMDA receptor itself. Our previous studies disclosed no differences in NMDA receptor subunit gene or splice isoform mRNA expression between control and kindled animals 28 days after the last kindled seizure. Here, we extend those earlier studies by measuring levels of subunit protein for NMDAR1, NR2A, and NR2B in the hippocampus of control and kindled animals, 28 days after the last kindled seizure. We report that kindling does not effect long-lasting changes in the levels of NMDA receptor subunit protein. Together these findings support the idea that alterations in NMDA receptor protein expression do not contribute to the novel properties of NMDA receptors induced by kindling.
Full Text
Duke Authors
Cited Authors
- Kraus, JE; McNamara, JO
Published Date
- October 30, 1998
Published In
Volume / Issue
- 61 / 1-2
Start / End Page
- 114 - 120
PubMed ID
- 9795176
Pubmed Central ID
- 9795176
International Standard Serial Number (ISSN)
- 0169-328X
Digital Object Identifier (DOI)
- 10.1016/s0169-328x(98)00220-4
Language
- eng
Conference Location
- Netherlands