A physical map across chromosome 11q22-q23 containing the major locus for ataxia telangiectasia.
Journal Article (Journal Article)
We have constructed a long-range physical map for 12 markers, including genes for GRIA4, IL1BC, and ACAT, across 9 Mb of chromosome 11q22-q23 in the region of the major locus for ataxia-telangiectasia (A-T). The markers fall into proximal and distal groups with respect to the centromere. We have linked the proximal and distal groups by hybridization to a 2.7-Mb NotI fragment and a 4.6-Mb MluI fragment. The following locus order was obtained: centromere-CJ52.75-J12.1C2-Y11B11R-IL1BC-+ ++hbcDNA-GRIA4-CJ52.3-Y11B29L-ACAT- CJ52.193-J12.8-Y11B06R-telomere. We show that hbcDNA/GRIA4 and CJ52.3 are very closely linked to each end, respectively, of the 2.7-Mb NotI fragment, thereby fixing the position of the complete contig. Our results indicate that the gene for A-T is flanked by the markers GRIA4 and J12.8, which are no more than 3 Mb apart, on a 4.6-Mb MluI fragment. The physical map allows rapid positioning of markers, and this will facilitate the construction of a YAC contig across the region.
Full Text
Duke Authors
Cited Authors
- Ambrose, HJ; Byrd, PJ; McConville, CM; Cooper, PR; Stankovic, T; Riley, JH; Shiloh, Y; McNamara, JO; Fukao, T; Taylor, AM
Published Date
- June 1994
Published In
Volume / Issue
- 21 / 3
Start / End Page
- 612 - 619
PubMed ID
- 7959739
International Standard Serial Number (ISSN)
- 0888-7543
Digital Object Identifier (DOI)
- 10.1006/geno.1994.1321
Language
- eng
Conference Location
- United States