Detection of Escherichia coli enterotoxins in stools.

Journal Article (Journal Article)

We determined whether enterotoxigenic Escherichia coli diarrhea could be diagnosed by direct examination of stools for heat-labile (LT) and heat-stable (ST) enterotoxins. The Y-1 adrenal cell and an enzyme-linked immunosorbent assay (ELISA) detected LT in 85 and 93%, respectively, of stool specimens obtained from adults with acute diarrhea from whom an LT- and ST-producing organism had been isolated. Furthermore, the ELISA assay detected LT in 8 of 35 stool specimens from which no LT-producing E. coli had been isolated. The infant mouse assay was utilized to detect ST in these stool specimens and was found to be an insensitive method, showing positive results in only 36% of the specimens from which an ST-producing organism was isolated. Further studies are warranted to determine the diagnostic value of direct detection of LT in stools, especially by the ELISA method.

Full Text

Duke Authors

Cited Authors

  • Merson, MH; Yolken, RH; Sack, RB; Froehlich, JL; Greenberg, HB; Huq, I; Black, RW

Published Date

  • July 1, 1980

Published In

Volume / Issue

  • 29 / 1

Start / End Page

  • 108 - 113

PubMed ID

  • 6995331

Pubmed Central ID

  • PMC551081

International Standard Serial Number (ISSN)

  • 0019-9567

Digital Object Identifier (DOI)

  • 10.1128/iai.29.1.108-113.1980


  • eng

Conference Location

  • United States