Comparison of four plating media for isolating Vibrio cholerae.

Journal Article (Journal Article)

Two brands of thiosulfate citrate bile salts sucrose agar and Monsur taurocholate tellurite gelatin (TTG) agar were compared with two newly developed media, sucrose tellurite teepol agar and Vibrio parahaemolyticus agar for isolation and identification of Vibrio cholerae. The thiosulfate citrate bile salts sucrose and TTG agars were the best selective media, whereas sucrose tellurite teepol agar was the poorest. Both TTG and sucrose tellurite teepol agars were good for use in follow-up serological tests, whereas only TTG agar could be used for follow-up oxidase tests. In our opinion TTG agar has more advantages for cholera research laboratories routinely culturing large numbers of patients for cholera on a daily basis and where media needs can be accurately predicted. In contrast, in smaller clinical laboratories or in laboratories investigating epidemics, thiosulfate citrate bile salts sucrose agar is best because it is commercially available and easy to prepare and can be used to distinguish colonies of suspect V. cholerae from V. parahaemolyticus.

Full Text

Duke Authors

Cited Authors

  • Morris, GK; Merson, MH; Huq, I; Kibrya, AK; Black, R

Published Date

  • January 1, 1979

Published In

Volume / Issue

  • 9 / 1

Start / End Page

  • 79 - 83

PubMed ID

  • 429540

Pubmed Central ID

  • PMC272960

International Standard Serial Number (ISSN)

  • 0095-1137

Digital Object Identifier (DOI)

  • 10.1128/jcm.9.1.79-83.1979


  • eng

Conference Location

  • United States