Skip to main content

Comparison of four plating media for isolating Vibrio cholerae.

Publication ,  Journal Article
Morris, GK; Merson, MH; Huq, I; Kibrya, AK; Black, R
Published in: J Clin Microbiol
January 1979

Two brands of thiosulfate citrate bile salts sucrose agar and Monsur taurocholate tellurite gelatin (TTG) agar were compared with two newly developed media, sucrose tellurite teepol agar and Vibrio parahaemolyticus agar for isolation and identification of Vibrio cholerae. The thiosulfate citrate bile salts sucrose and TTG agars were the best selective media, whereas sucrose tellurite teepol agar was the poorest. Both TTG and sucrose tellurite teepol agars were good for use in follow-up serological tests, whereas only TTG agar could be used for follow-up oxidase tests. In our opinion TTG agar has more advantages for cholera research laboratories routinely culturing large numbers of patients for cholera on a daily basis and where media needs can be accurately predicted. In contrast, in smaller clinical laboratories or in laboratories investigating epidemics, thiosulfate citrate bile salts sucrose agar is best because it is commercially available and easy to prepare and can be used to distinguish colonies of suspect V. cholerae from V. parahaemolyticus.

Duke Scholars

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

January 1979

Volume

9

Issue

1

Start / End Page

79 / 83

Location

United States

Related Subject Headings

  • Vibrio cholerae
  • Thiosulfates
  • Tellurium
  • Microbiology
  • Humans
  • Feces
  • Culture Media
  • Citrates
  • Bangladesh
  • 3207 Medical microbiology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Morris, G. K., Merson, M. H., Huq, I., Kibrya, A. K., & Black, R. (1979). Comparison of four plating media for isolating Vibrio cholerae. J Clin Microbiol, 9(1), 79–83. https://doi.org/10.1128/jcm.9.1.79-83.1979
Morris, G. K., M. H. Merson, I. Huq, A. K. Kibrya, and R. Black. “Comparison of four plating media for isolating Vibrio cholerae.J Clin Microbiol 9, no. 1 (January 1979): 79–83. https://doi.org/10.1128/jcm.9.1.79-83.1979.
Morris GK, Merson MH, Huq I, Kibrya AK, Black R. Comparison of four plating media for isolating Vibrio cholerae. J Clin Microbiol. 1979 Jan;9(1):79–83.
Morris, G. K., et al. “Comparison of four plating media for isolating Vibrio cholerae.J Clin Microbiol, vol. 9, no. 1, Jan. 1979, pp. 79–83. Pubmed, doi:10.1128/jcm.9.1.79-83.1979.
Morris GK, Merson MH, Huq I, Kibrya AK, Black R. Comparison of four plating media for isolating Vibrio cholerae. J Clin Microbiol. 1979 Jan;9(1):79–83.

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

January 1979

Volume

9

Issue

1

Start / End Page

79 / 83

Location

United States

Related Subject Headings

  • Vibrio cholerae
  • Thiosulfates
  • Tellurium
  • Microbiology
  • Humans
  • Feces
  • Culture Media
  • Citrates
  • Bangladesh
  • 3207 Medical microbiology