Use of colony pools for diagnosis of enterotoxigenic Escherichia coli diarrhea.

Journal Article (Journal Article)

Diagnosis of enterotoxigenic Escherichia coli diarrhea was made in 109 adult males with an acute dehydrating cholera-like syndrome in Dacca, Bangladesh, by testing 10 colonies isolated from admission stool specimens for production of heat-labile and heat-stable toxins. Toxin testing of one colony yielded a diagnosis in 92% of the cases, testing of two colonies yielded a diagnosis in 95% of the cases, testing of a pool of 5 colonies yielded a diagnosis in 95% of the cases, and testing of a pool of 10 colonies yielded a diagnosis in 96% of the cases. From stool cultures obtained on subsequent days, toxin testing of individual colonies and pools revealed diminished efficacy of pooling with decreasing numbers of enterotoxin-positive isolates in the pool. To detect the presence of enterotoxigenic E. coli in stools, toxin testing of 5 individual isolates and a pool of 10 colonies was found to be almost as effective as the testing of 10 individual isolates.

Full Text

Duke Authors

Cited Authors

  • Merson, MH; Sack, RB; Kibriya, AK; Al-Mahmood, A; Adamed, QS; Huq, I

Published Date

  • April 1, 1979

Published In

Volume / Issue

  • 9 / 4

Start / End Page

  • 493 - 497

PubMed ID

  • 379039

Pubmed Central ID

  • PMC273061

International Standard Serial Number (ISSN)

  • 0095-1137

Digital Object Identifier (DOI)

  • 10.1128/jcm.9.4.493-497.1979


  • eng

Conference Location

  • United States