Use of colony pools for diagnosis of enterotoxigenic Escherichia coli diarrhea.
Journal Article (Journal Article)
Diagnosis of enterotoxigenic Escherichia coli diarrhea was made in 109 adult males with an acute dehydrating cholera-like syndrome in Dacca, Bangladesh, by testing 10 colonies isolated from admission stool specimens for production of heat-labile and heat-stable toxins. Toxin testing of one colony yielded a diagnosis in 92% of the cases, testing of two colonies yielded a diagnosis in 95% of the cases, testing of a pool of 5 colonies yielded a diagnosis in 95% of the cases, and testing of a pool of 10 colonies yielded a diagnosis in 96% of the cases. From stool cultures obtained on subsequent days, toxin testing of individual colonies and pools revealed diminished efficacy of pooling with decreasing numbers of enterotoxin-positive isolates in the pool. To detect the presence of enterotoxigenic E. coli in stools, toxin testing of 5 individual isolates and a pool of 10 colonies was found to be almost as effective as the testing of 10 individual isolates.
Full Text
Duke Authors
Cited Authors
- Merson, MH; Sack, RB; Kibriya, AK; Al-Mahmood, A; Adamed, QS; Huq, I
Published Date
- April 1, 1979
Published In
Volume / Issue
- 9 / 4
Start / End Page
- 493 - 497
PubMed ID
- 379039
Pubmed Central ID
- PMC273061
International Standard Serial Number (ISSN)
- 0095-1137
Digital Object Identifier (DOI)
- 10.1128/jcm.9.4.493-497.1979
Language
- eng
Conference Location
- United States