Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin.
Journal Article (Journal Article)
The development of an enzyme-linked immunosorbent assay (ELISA) for the detection of heat-labile Escherichia coli enterotoxin is described. The assay, which is based on the immunological similarity between Vibrio cholerae toxin and heat-labile E. coli enterotoxin, is similar in design to a radioimmunoassay but utilizes enzyme-labeled rather than radioactive isotope-labeled reagents. The ELISA system is as sensitive as both radioimmunoassay and the y-1 adrenal cell assay for the detection of heat-labile E. coli enterotoxin but requires neither radioactive reagents nor tissue culture techniques. The ELISA is easy to perform and is adaptable for use in small laboratories.
Full Text
Duke Authors
Cited Authors
- Yolken, RH; Greenberg, HB; Merson, MH; Sack, RB; Kapikian, AZ
Published Date
- November 1977
Published In
Volume / Issue
- 6 / 5
Start / End Page
- 439 - 444
PubMed ID
- 336638
Pubmed Central ID
- PMC274794
International Standard Serial Number (ISSN)
- 0095-1137
Digital Object Identifier (DOI)
- 10.1128/jcm.6.5.439-444.1977
Language
- eng
Conference Location
- United States