Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin.
Publication
, Journal Article
Yolken, RH; Greenberg, HB; Merson, MH; Sack, RB; Kapikian, AZ
Published in: J Clin Microbiol
November 1977
The development of an enzyme-linked immunosorbent assay (ELISA) for the detection of heat-labile Escherichia coli enterotoxin is described. The assay, which is based on the immunological similarity between Vibrio cholerae toxin and heat-labile E. coli enterotoxin, is similar in design to a radioimmunoassay but utilizes enzyme-labeled rather than radioactive isotope-labeled reagents. The ELISA system is as sensitive as both radioimmunoassay and the y-1 adrenal cell assay for the detection of heat-labile E. coli enterotoxin but requires neither radioactive reagents nor tissue culture techniques. The ELISA is easy to perform and is adaptable for use in small laboratories.
Duke Scholars
Altmetric Attention Stats
Dimensions Citation Stats
Published In
J Clin Microbiol
DOI
ISSN
0095-1137
Publication Date
November 1977
Volume
6
Issue
5
Start / End Page
439 / 444
Location
United States
Related Subject Headings
- Microbiology
- Immunoenzyme Techniques
- Humans
- Hot Temperature
- Feces
- Escherichia coli
- Enzyme-Linked Immunosorbent Assay
- Enterotoxins
- Drug Stability
- 3207 Medical microbiology
Citation
APA
Chicago
ICMJE
MLA
NLM
Yolken, R. H., Greenberg, H. B., Merson, M. H., Sack, R. B., & Kapikian, A. Z. (1977). Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin. J Clin Microbiol, 6(5), 439–444. https://doi.org/10.1128/jcm.6.5.439-444.1977
Yolken, R. H., H. B. Greenberg, M. H. Merson, R. B. Sack, and A. Z. Kapikian. “Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin.” J Clin Microbiol 6, no. 5 (November 1977): 439–44. https://doi.org/10.1128/jcm.6.5.439-444.1977.
Yolken RH, Greenberg HB, Merson MH, Sack RB, Kapikian AZ. Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin. J Clin Microbiol. 1977 Nov;6(5):439–44.
Yolken, R. H., et al. “Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin.” J Clin Microbiol, vol. 6, no. 5, Nov. 1977, pp. 439–44. Pubmed, doi:10.1128/jcm.6.5.439-444.1977.
Yolken RH, Greenberg HB, Merson MH, Sack RB, Kapikian AZ. Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin. J Clin Microbiol. 1977 Nov;6(5):439–444.
Published In
J Clin Microbiol
DOI
ISSN
0095-1137
Publication Date
November 1977
Volume
6
Issue
5
Start / End Page
439 / 444
Location
United States
Related Subject Headings
- Microbiology
- Immunoenzyme Techniques
- Humans
- Hot Temperature
- Feces
- Escherichia coli
- Enzyme-Linked Immunosorbent Assay
- Enterotoxins
- Drug Stability
- 3207 Medical microbiology