Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin.

Published

Journal Article

The development of an enzyme-linked immunosorbent assay (ELISA) for the detection of heat-labile Escherichia coli enterotoxin is described. The assay, which is based on the immunological similarity between Vibrio cholerae toxin and heat-labile E. coli enterotoxin, is similar in design to a radioimmunoassay but utilizes enzyme-labeled rather than radioactive isotope-labeled reagents. The ELISA system is as sensitive as both radioimmunoassay and the y-1 adrenal cell assay for the detection of heat-labile E. coli enterotoxin but requires neither radioactive reagents nor tissue culture techniques. The ELISA is easy to perform and is adaptable for use in small laboratories.

Full Text

Duke Authors

Cited Authors

  • Yolken, RH; Greenberg, HB; Merson, MH; Sack, RB; Kapikian, AZ

Published Date

  • November 1977

Published In

Volume / Issue

  • 6 / 5

Start / End Page

  • 439 - 444

PubMed ID

  • 336638

Pubmed Central ID

  • 336638

International Standard Serial Number (ISSN)

  • 0095-1137

Language

  • eng

Conference Location

  • United States