An LC-MS/MS method for determination of glycosaminoglycan content of urine for the diagnosis of mucopolysaccharidoses


Journal Article

A quantitative liquid chromatography/mass spectrometry/mass spectrometry (LC/MS/MS) is developed to measure the characterstic disaccharide derived from glycosaminoglycans (GAG) by partial methanolysis. The results show that methanolysis replace the sulphate groups by hydroxyl groups and methylates. The polymeric GAG collapse to a common disaccharide, the sodiated adduct of which dissociates to a monosaccharide by CID. The measured GAG concentrations were normalized by urinary creatinine content.

Duke Authors

Cited Authors

  • An, Y; Stevens, RD; Muenzer, J; Clarke, JTR; Millington, DS

Published Date

  • December 1, 2002

Published In

  • Proceedings 50th Asms Conference on Mass Spectrometry and Allied Topics

Start / End Page

  • 597 - 598

Citation Source

  • Scopus