Modulation of the unfolded protein response by GRP78 in prostate cancer.

Journal Article (Journal Article;Review)

The unfolded protein response (UPR) is an adaptive survival mechanism through which cells can weather the stress of misfolded protein accumulation induced by a wide variety of pathophysiologic and pharmacologic insults. The ER chaperone GRP78 is a central modulator of the UPR both through its protein-binding capacity and its direct regulation of the UPR signaling molecules IRE1α, PERK, and ATF6. Recent reports have revealed the presence of GRP78 on the surface of cancer cells. Biological roles for cell-surface GRP78 include competing NH(2)-domain and COOH-domain agonist receptor activities that induce opposite effects on proliferation and apoptosis. Modulation of the UPR impacts both of these processes directly and indirectly. Here, we outline methods that we use to investigate UPR modulation via direct ligation of cell-surface GRP78. Specifically, we review methods of cell culture, cell-signaling analysis with emphasis on UPR components, and ultimately, the impact that these have on cell proliferation, survival, and apoptosis.

Full Text

Duke Authors

Cited Authors

  • de Ridder, G; Ray, R; Misra, UK; Pizzo, SV

Published Date

  • 2011

Published In

Volume / Issue

  • 489 /

Start / End Page

  • 245 - 257

PubMed ID

  • 21266234

Electronic International Standard Serial Number (EISSN)

  • 1557-7988

Digital Object Identifier (DOI)

  • 10.1016/B978-0-12-385116-1.00014-5


  • eng

Conference Location

  • United States