Analysis of the excision step in human DNA mismatch repair.

Journal Article (Journal Article)

The reaction responsible for replication error correction by mismatch repair proceeds via several steps: mismatch recognition, mismatch-provoked excision, repair DNA synthesis, and ligation. Key steps in this process are the recognition and subsequent exonucleolytic removal of the mispair. A minimal system comprised of human MutSalpha (MSH2*MSH6), MutLalpha (MLH1*PMS2), exonuclease I (EXOI), replication protein A (RPA), proliferating cell nuclear antigen (PCNA), and replication factor C (RFC) is sufficient to support mismatch-provoked excision in vitro. This chapter describes methods for analysis of the reconstituted excision reaction.

Full Text

Duke Authors

Cited Authors

  • Genschel, J; Modrich, P

Published Date

  • 2006

Published In

Volume / Issue

  • 408 /

Start / End Page

  • 273 - 284

PubMed ID

  • 16793375

International Standard Serial Number (ISSN)

  • 0076-6879

Digital Object Identifier (DOI)

  • 10.1016/S0076-6879(06)08017-7


  • eng

Conference Location

  • United States