Analysis of the excision step in human DNA mismatch repair.
Journal Article (Journal Article)
The reaction responsible for replication error correction by mismatch repair proceeds via several steps: mismatch recognition, mismatch-provoked excision, repair DNA synthesis, and ligation. Key steps in this process are the recognition and subsequent exonucleolytic removal of the mispair. A minimal system comprised of human MutSalpha (MSH2*MSH6), MutLalpha (MLH1*PMS2), exonuclease I (EXOI), replication protein A (RPA), proliferating cell nuclear antigen (PCNA), and replication factor C (RFC) is sufficient to support mismatch-provoked excision in vitro. This chapter describes methods for analysis of the reconstituted excision reaction.
Full Text
Duke Authors
Cited Authors
- Genschel, J; Modrich, P
Published Date
- 2006
Published In
Volume / Issue
- 408 /
Start / End Page
- 273 - 284
PubMed ID
- 16793375
International Standard Serial Number (ISSN)
- 0076-6879
Digital Object Identifier (DOI)
- 10.1016/S0076-6879(06)08017-7
Language
- eng
Conference Location
- United States