Analysis of the excision step in human DNA mismatch repair.
Publication
, Journal Article
Genschel, J; Modrich, P
Published in: Methods Enzymol
2006
The reaction responsible for replication error correction by mismatch repair proceeds via several steps: mismatch recognition, mismatch-provoked excision, repair DNA synthesis, and ligation. Key steps in this process are the recognition and subsequent exonucleolytic removal of the mispair. A minimal system comprised of human MutSalpha (MSH2*MSH6), MutLalpha (MLH1*PMS2), exonuclease I (EXOI), replication protein A (RPA), proliferating cell nuclear antigen (PCNA), and replication factor C (RFC) is sufficient to support mismatch-provoked excision in vitro. This chapter describes methods for analysis of the reconstituted excision reaction.
Duke Scholars
Published In
Methods Enzymol
DOI
ISSN
0076-6879
Publication Date
2006
Volume
408
Start / End Page
273 / 284
Location
United States
Related Subject Headings
- Replication Protein C
- Replication Protein A
- Proliferating Cell Nuclear Antigen
- Neoplasm Proteins
- MutS Homolog 2 Protein
- MutL Proteins
- Humans
- Exodeoxyribonucleases
- DNA-Binding Proteins
- DNA Repair Enzymes
Citation
APA
Chicago
ICMJE
MLA
NLM
Genschel, J., & Modrich, P. (2006). Analysis of the excision step in human DNA mismatch repair. Methods Enzymol, 408, 273–284. https://doi.org/10.1016/S0076-6879(06)08017-7
Genschel, Jochen, and Paul Modrich. “Analysis of the excision step in human DNA mismatch repair.” Methods Enzymol 408 (2006): 273–84. https://doi.org/10.1016/S0076-6879(06)08017-7.
Genschel J, Modrich P. Analysis of the excision step in human DNA mismatch repair. Methods Enzymol. 2006;408:273–84.
Genschel, Jochen, and Paul Modrich. “Analysis of the excision step in human DNA mismatch repair.” Methods Enzymol, vol. 408, 2006, pp. 273–84. Pubmed, doi:10.1016/S0076-6879(06)08017-7.
Genschel J, Modrich P. Analysis of the excision step in human DNA mismatch repair. Methods Enzymol. 2006;408:273–284.
Published In
Methods Enzymol
DOI
ISSN
0076-6879
Publication Date
2006
Volume
408
Start / End Page
273 / 284
Location
United States
Related Subject Headings
- Replication Protein C
- Replication Protein A
- Proliferating Cell Nuclear Antigen
- Neoplasm Proteins
- MutS Homolog 2 Protein
- MutL Proteins
- Humans
- Exodeoxyribonucleases
- DNA-Binding Proteins
- DNA Repair Enzymes