High rate of CAD gene amplification in human cells deficient in MLH1 or MSH6.

Journal Article (Journal Article)

MutS and MutL homologs have been implicated in multiple genetic stabilization pathways. The activities participate in the correction of DNA biosynthetic errors, are involved in cellular responses to certain types of DNA damage, and serve to ensure the fidelity of genetic recombination. We show here that the rate of CAD (carbamyl-P synthetase/aspartate transcarbamylase/dihydroorotase) gene amplification is elevated 50- to 100-fold in human cell lines deficient in MLH1 or MSH6, as compared with mismatch repair-proficient control cells. Fluorescence in situ hybridization indicates that these amplification events are the probable consequence of unequal sister chromatid exchanges involving chromosome 2, as well as translocation events involving other chromosomes. These results implicate MutS alpha and MutL alpha in the suppression of gene amplification and suggest that defects in this genetic stabilization function may contribute to the cancer predisposition associated with mismatch repair deficiency.

Full Text

Duke Authors

Cited Authors

  • Chen, S; Bigner, SH; Modrich, P

Published Date

  • November 20, 2001

Published In

Volume / Issue

  • 98 / 24

Start / End Page

  • 13802 - 13807

PubMed ID

  • 11717437

Pubmed Central ID

  • PMC61122

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.241508098


  • eng

Conference Location

  • United States