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Mismatch-, MutS-, MutL-, and helicase II-dependent unwinding from the single-strand break of an incised heteroduplex.

Publication ,  Journal Article
Dao, V; Modrich, P
Published in: J Biol Chem
April 10, 1998

Escherichia coli MutS, MutL, and DNA helicase II are sufficient to initiate mismatch-dependent unwinding of an incised heteroduplex (Yamaguchi, M., Dao, V., and Modrich, P. (1998) J. Biol. Chem., 273, 9197-9201). We have studied unwinding of 6.4-kilobase circular G-T heteroduplexes that contain a single-strand incision, 808 base pairs 5' to the mismatch or 1023 base pairs 3' to the mispair as viewed along the shorter path between the two DNA sites. Unwinding of both substrates in the presence of MutS, MutL, DNA helicase II, and single-stranded DNA binding protein was mismatch-dependent and initiated at the single-strand break. Although unwinding occurred in both directions from the strand break, it was biased toward the shorter path linking the strand break and the mispair. MutS and MutL are thus sufficient to coordinate mismatch recognition to the orientation-dependent activation of helicase II unwinding at a single-strand break located a kilobase from the mispair.

Duke Scholars

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

April 10, 1998

Volume

273

Issue

15

Start / End Page

9202 / 9207

Location

United States

Related Subject Headings

  • Oligonucleotide Probes
  • Nucleic Acid Heteroduplexes
  • Nucleic Acid Conformation
  • MutS DNA Mismatch-Binding Protein
  • MutL Proteins
  • Models, Molecular
  • Kinetics
  • Escherichia coli Proteins
  • Escherichia coli
  • DNA-Binding Proteins
 

Citation

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Dao, V., & Modrich, P. (1998). Mismatch-, MutS-, MutL-, and helicase II-dependent unwinding from the single-strand break of an incised heteroduplex. J Biol Chem, 273(15), 9202–9207. https://doi.org/10.1074/jbc.273.15.9202
Dao, V., and P. Modrich. “Mismatch-, MutS-, MutL-, and helicase II-dependent unwinding from the single-strand break of an incised heteroduplex.J Biol Chem 273, no. 15 (April 10, 1998): 9202–7. https://doi.org/10.1074/jbc.273.15.9202.
Dao, V., and P. Modrich. “Mismatch-, MutS-, MutL-, and helicase II-dependent unwinding from the single-strand break of an incised heteroduplex.J Biol Chem, vol. 273, no. 15, Apr. 1998, pp. 9202–07. Pubmed, doi:10.1074/jbc.273.15.9202.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

April 10, 1998

Volume

273

Issue

15

Start / End Page

9202 / 9207

Location

United States

Related Subject Headings

  • Oligonucleotide Probes
  • Nucleic Acid Heteroduplexes
  • Nucleic Acid Conformation
  • MutS DNA Mismatch-Binding Protein
  • MutL Proteins
  • Models, Molecular
  • Kinetics
  • Escherichia coli Proteins
  • Escherichia coli
  • DNA-Binding Proteins