Increased transversions in a novel mutator colon cancer cell line.

Journal Article (Journal Article)

We describe a novel mutator phenotype in the Vaco411 colon cancer cell line which increases the spontaneous mutation rate 10-100-fold over background. This mutator results primarily in transversion base substitutions which are found infrequently in repair competent cells. Of the four possible types of transversions, only three were principally recovered. Spontaneous mutations recovered also included transitions and large deletions, but very few frameshifts were recovered. When compared to known mismatch repair defective colon cancer mutators, the distribution of mutations in Vaco411 is significantly different. Consistent with this difference, Vaco411 extracts are proficient in assays of mismatch repair. The Vaco411 mutator appears to be novel, and is not an obvious human homologue of any of the previously characterized bacterial or yeast transversion phenotypes. Several hypotheses by which this mutator may produce transversions are presented.

Full Text

Duke Authors

Cited Authors

  • Eshleman, JR; Donover, PS; Littman, SJ; Swinler, SE; Li, GM; Lutterbaugh, JD; Willson, JK; Modrich, P; Sedwick, WD; Markowitz, SD; Veigl, ML

Published Date

  • March 5, 1998

Published In

Volume / Issue

  • 16 / 9

Start / End Page

  • 1125 - 1130

PubMed ID

  • 9528854

International Standard Serial Number (ISSN)

  • 0950-9232

Digital Object Identifier (DOI)

  • 10.1038/sj.onc.1201629


  • eng

Conference Location

  • England