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Increased transversions in a novel mutator colon cancer cell line.

Publication ,  Journal Article
Eshleman, JR; Donover, PS; Littman, SJ; Swinler, SE; Li, GM; Lutterbaugh, JD; Willson, JK; Modrich, P; Sedwick, WD; Markowitz, SD; Veigl, ML
Published in: Oncogene
March 5, 1998

We describe a novel mutator phenotype in the Vaco411 colon cancer cell line which increases the spontaneous mutation rate 10-100-fold over background. This mutator results primarily in transversion base substitutions which are found infrequently in repair competent cells. Of the four possible types of transversions, only three were principally recovered. Spontaneous mutations recovered also included transitions and large deletions, but very few frameshifts were recovered. When compared to known mismatch repair defective colon cancer mutators, the distribution of mutations in Vaco411 is significantly different. Consistent with this difference, Vaco411 extracts are proficient in assays of mismatch repair. The Vaco411 mutator appears to be novel, and is not an obvious human homologue of any of the previously characterized bacterial or yeast transversion phenotypes. Several hypotheses by which this mutator may produce transversions are presented.

Duke Scholars

Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

March 5, 1998

Volume

16

Issue

9

Start / End Page

1125 / 1130

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Sequence Deletion
  • Point Mutation
  • Oncology & Carcinogenesis
  • Mutation
  • Hypoxanthine Phosphoribosyltransferase
  • Humans
  • DNA Repair
  • Colonic Neoplasms
  • Cell Line
 

Citation

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Eshleman, J. R., Donover, P. S., Littman, S. J., Swinler, S. E., Li, G. M., Lutterbaugh, J. D., … Veigl, M. L. (1998). Increased transversions in a novel mutator colon cancer cell line. Oncogene, 16(9), 1125–1130. https://doi.org/10.1038/sj.onc.1201629
Eshleman, J. R., P. S. Donover, S. J. Littman, S. E. Swinler, G. M. Li, J. D. Lutterbaugh, J. K. Willson, et al. “Increased transversions in a novel mutator colon cancer cell line.Oncogene 16, no. 9 (March 5, 1998): 1125–30. https://doi.org/10.1038/sj.onc.1201629.
Eshleman JR, Donover PS, Littman SJ, Swinler SE, Li GM, Lutterbaugh JD, et al. Increased transversions in a novel mutator colon cancer cell line. Oncogene. 1998 Mar 5;16(9):1125–30.
Eshleman, J. R., et al. “Increased transversions in a novel mutator colon cancer cell line.Oncogene, vol. 16, no. 9, Mar. 1998, pp. 1125–30. Pubmed, doi:10.1038/sj.onc.1201629.
Eshleman JR, Donover PS, Littman SJ, Swinler SE, Li GM, Lutterbaugh JD, Willson JK, Modrich P, Sedwick WD, Markowitz SD, Veigl ML. Increased transversions in a novel mutator colon cancer cell line. Oncogene. 1998 Mar 5;16(9):1125–1130.

Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

March 5, 1998

Volume

16

Issue

9

Start / End Page

1125 / 1130

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Sequence Deletion
  • Point Mutation
  • Oncology & Carcinogenesis
  • Mutation
  • Hypoxanthine Phosphoribosyltransferase
  • Humans
  • DNA Repair
  • Colonic Neoplasms
  • Cell Line