Mapping individual cosmid DNAs by direct AFM imaging.

Published

Journal Article

Individual cosmid clones have been restriction mapped by directly imaging, with the atomic force microscope (AFM), a mutant EcoRI endonuclease site-specifically bound to DNA. Images and data are presented that locate six restriction sites, predicted from gel electrophoresis, on a 35-kb cosmid isolated from mouse chromosome 7. Measured distances between endonuclease molecules bound to lambda DNA, when compared to known values, demonstrate the accuracy of AFM mapping to better than 1%. These results may be extended to identify other important site-specific protein-DNA interactions, such as transcription factor and mismatch repair enzyme binding, difficult to resolve by current techniques.

Full Text

Duke Authors

Cited Authors

  • Allison, DP; Kerper, PS; Doktycz, MJ; Thundat, T; Modrich, P; Larimer, FW; Johnson, DK; Hoyt, PR; Mucenski, ML; Warmack, RJ

Published Date

  • May 1, 1997

Published In

Volume / Issue

  • 41 / 3

Start / End Page

  • 379 - 384

PubMed ID

  • 9169135

Pubmed Central ID

  • 9169135

International Standard Serial Number (ISSN)

  • 0888-7543

Digital Object Identifier (DOI)

  • 10.1006/geno.1997.4686

Language

  • eng

Conference Location

  • United States