DNA polymerase delta is required for human mismatch repair in vitro.

Published

Journal Article

HeLa nuclear extract was resolved into a depleted fraction incapable of supporting mismatch repair in vitro, and repair activity was restored upon the addition of a purified fraction isolated from HeLa cells by in vitro complementation assay. The highly enriched complementing activity copurified with a DNA polymerase, and the most pure fraction contained DNA polymerase delta but was free of detectable DNA polymerases alpha and epsilon. Calf thymus DNA polymerase delta also fully restored mismatch repair to the depleted extract, indicating DNA polymerase delta is required for mismatch repair in human cells. However, due to the presence of DNA polymerases alpha and epsilon in the depleted extract, potential involvement of one or both of these activities in the reaction cannot be excluded.

Full Text

Duke Authors

Cited Authors

  • Longley, MJ; Pierce, AJ; Modrich, P

Published Date

  • April 18, 1997

Published In

Volume / Issue

  • 272 / 16

Start / End Page

  • 10917 - 10921

PubMed ID

  • 9099749

Pubmed Central ID

  • 9099749

International Standard Serial Number (ISSN)

  • 0021-9258

Digital Object Identifier (DOI)

  • 10.1074/jbc.272.16.10917

Language

  • eng

Conference Location

  • United States