Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs.


Journal Article

The Escherichia coli mutS gene product is involved in mismatch correction in this organism. We have purified a biologically active form of the 97,000 Mr protein to near homogeneity from an overproducing strain. Enzymatic and chemical protection ("footprinting") experiments have demonstrated that mutS-encoded protein specifically binds to DNA regions containing a single base-pair mismatch. The protein displayed variable affinity for the limited set of mismatches tested (G-T greater than G-A approximately equal to A-C greater than T-C).

Full Text

Duke Authors

Cited Authors

  • Su, SS; Modrich, P

Published Date

  • July 1, 1986

Published In

Volume / Issue

  • 83 / 14

Start / End Page

  • 5057 - 5061

PubMed ID

  • 3014530

Pubmed Central ID

  • 3014530

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.83.14.5057


  • eng

Conference Location

  • United States