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Positive-selection cloning vehicle useful for overproduction of hybrid proteins.

Publication ,  Journal Article
Cheng, SC; Modrich, P
Published in: J Bacteriol
May 1983

Plasmid pSCC31 contains the EcoRI endonuclease gene downstream from lambda pL. It does not yield transformants upon introduction into Escherichia coli unless the structural integrity of the endonuclease is destroyed. This makes it useful as a positive-selection cloning vehicle which can be employed for regulated overproduction of hybrid proteins.

Duke Scholars

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

May 1983

Volume

154

Issue

2

Start / End Page

1005 / 1008

Location

United States

Related Subject Headings

  • Viral Proteins
  • Protein Biosynthesis
  • Plasmids
  • Microbiology
  • Genetic Vectors
  • Genes, Viral
  • Genes
  • Escherichia coli
  • Deoxyribonuclease EcoRI
  • DNA Restriction Enzymes
 

Citation

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MLA
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Cheng, S. C., & Modrich, P. (1983). Positive-selection cloning vehicle useful for overproduction of hybrid proteins. J Bacteriol, 154(2), 1005–1008. https://doi.org/10.1128/jb.154.2.1005-1008.1983
Cheng, S. C., and P. Modrich. “Positive-selection cloning vehicle useful for overproduction of hybrid proteins.J Bacteriol 154, no. 2 (May 1983): 1005–8. https://doi.org/10.1128/jb.154.2.1005-1008.1983.
Cheng SC, Modrich P. Positive-selection cloning vehicle useful for overproduction of hybrid proteins. J Bacteriol. 1983 May;154(2):1005–8.
Cheng, S. C., and P. Modrich. “Positive-selection cloning vehicle useful for overproduction of hybrid proteins.J Bacteriol, vol. 154, no. 2, May 1983, pp. 1005–08. Pubmed, doi:10.1128/jb.154.2.1005-1008.1983.
Cheng SC, Modrich P. Positive-selection cloning vehicle useful for overproduction of hybrid proteins. J Bacteriol. 1983 May;154(2):1005–1008.

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

May 1983

Volume

154

Issue

2

Start / End Page

1005 / 1008

Location

United States

Related Subject Headings

  • Viral Proteins
  • Protein Biosynthesis
  • Plasmids
  • Microbiology
  • Genetic Vectors
  • Genes, Viral
  • Genes
  • Escherichia coli
  • Deoxyribonuclease EcoRI
  • DNA Restriction Enzymes