Positive-selection cloning vehicle useful for overproduction of hybrid proteins.

Journal Article (Journal Article)

Plasmid pSCC31 contains the EcoRI endonuclease gene downstream from lambda pL. It does not yield transformants upon introduction into Escherichia coli unless the structural integrity of the endonuclease is destroyed. This makes it useful as a positive-selection cloning vehicle which can be employed for regulated overproduction of hybrid proteins.

Full Text

Duke Authors

Cited Authors

  • Cheng, SC; Modrich, P

Published Date

  • May 1983

Published In

Volume / Issue

  • 154 / 2

Start / End Page

  • 1005 - 1008

PubMed ID

  • 6302074

Pubmed Central ID

  • PMC217560

International Standard Serial Number (ISSN)

  • 0021-9193

Digital Object Identifier (DOI)

  • 10.1128/jb.154.2.1005-1008.1983


  • eng

Conference Location

  • United States