Positive-selection cloning vehicle useful for overproduction of hybrid proteins.
Journal Article (Journal Article)
Plasmid pSCC31 contains the EcoRI endonuclease gene downstream from lambda pL. It does not yield transformants upon introduction into Escherichia coli unless the structural integrity of the endonuclease is destroyed. This makes it useful as a positive-selection cloning vehicle which can be employed for regulated overproduction of hybrid proteins.
Full Text
Duke Authors
Cited Authors
- Cheng, SC; Modrich, P
Published Date
- May 1, 1983
Published In
Volume / Issue
- 154 / 2
Start / End Page
- 1005 - 1008
PubMed ID
- 6302074
Pubmed Central ID
- 6302074
International Standard Serial Number (ISSN)
- 0021-9193
Digital Object Identifier (DOI)
- 10.1128/JB.154.2.1005-1008.1983
Language
- eng
Conference Location
- United States