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Stereochemical course of nucleotidyl transfer catalyzed by bacteriophage T7 induced DNA polymerase.

Publication ,  Journal Article
Brody, RS; Adler, S; Modrich, P; Stec, WJ; Leznikowski, ZJ; Frey, PA
Published in: Biochemistry
May 11, 1982

The bacteriophage T7 induced DNA polymerase, consisting of the phage specified gene 5 protein associated with Escherichia coli thioredoxin, catalyzes the copolymerization of SP-dATP alpha S with dTTP, producing the alternating of polymer poly[dTs-A)] by a mechanism involving inversion of configuration at P alpha. Degradation of poly[d(5s-A)] by the nucleolytic action of E. coli DNA polymerase produced the dinucleotide pdTps-dA, whose configuration at the phosphorothioate diester was assigned as R by comparison of the phosphorus-31 nuclear magnetic resonance chemical shift (55.0 ppm downfield from H3PO4) with that of an authentic sample. Further degradation by alkaline phosphatase to Rp-dTps-dA (55.6 ppm downfield from H3PO4) confirmed the configuration. The stereochemistry provides no evidence of a double displacement mechanism.

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Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

May 11, 1982

Volume

21

Issue

10

Start / End Page

2570 / 2572

Location

United States

Related Subject Headings

  • Thioredoxins
  • Thionucleotides
  • T-Phages
  • Stereoisomerism
  • Phosphoproteins
  • Models, Chemical
  • Deoxyribonucleotides
  • Deoxyadenine Nucleotides
  • DNA-Directed DNA Polymerase
  • Biochemistry & Molecular Biology
 

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Brody, R. S., Adler, S., Modrich, P., Stec, W. J., Leznikowski, Z. J., & Frey, P. A. (1982). Stereochemical course of nucleotidyl transfer catalyzed by bacteriophage T7 induced DNA polymerase. Biochemistry, 21(10), 2570–2572. https://doi.org/10.1021/bi00539a042
Brody, R. S., S. Adler, P. Modrich, W. J. Stec, Z. J. Leznikowski, and P. A. Frey. “Stereochemical course of nucleotidyl transfer catalyzed by bacteriophage T7 induced DNA polymerase.Biochemistry 21, no. 10 (May 11, 1982): 2570–72. https://doi.org/10.1021/bi00539a042.
Brody RS, Adler S, Modrich P, Stec WJ, Leznikowski ZJ, Frey PA. Stereochemical course of nucleotidyl transfer catalyzed by bacteriophage T7 induced DNA polymerase. Biochemistry. 1982 May 11;21(10):2570–2.
Brody, R. S., et al. “Stereochemical course of nucleotidyl transfer catalyzed by bacteriophage T7 induced DNA polymerase.Biochemistry, vol. 21, no. 10, May 1982, pp. 2570–72. Pubmed, doi:10.1021/bi00539a042.
Brody RS, Adler S, Modrich P, Stec WJ, Leznikowski ZJ, Frey PA. Stereochemical course of nucleotidyl transfer catalyzed by bacteriophage T7 induced DNA polymerase. Biochemistry. 1982 May 11;21(10):2570–2572.
Journal cover image

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

May 11, 1982

Volume

21

Issue

10

Start / End Page

2570 / 2572

Location

United States

Related Subject Headings

  • Thioredoxins
  • Thionucleotides
  • T-Phages
  • Stereoisomerism
  • Phosphoproteins
  • Models, Chemical
  • Deoxyribonucleotides
  • Deoxyadenine Nucleotides
  • DNA-Directed DNA Polymerase
  • Biochemistry & Molecular Biology