Substrate dependence of the mechanism of EcoRI endonuclease.

Published

Journal Article

The mechanism of EcoRI endonuclease is substrate dependent. At 37 degrees dissociation of the enzyme-Form II DNA intermediates of ColE1 DNA and bacteriophage G4 RFI DNA is negligible. Therefore, both DNA strands with in the EcoRI sequence are cleaved during a single binding event. However, double strand cleavage of SV40 DNA occurs without dissociation of the enzyme in only 75% of the catalytic events. This mechanistic difference presumably reflects sequence differences about the EcoRI sites of these DNA's.

Full Text

Duke Authors

Cited Authors

  • Rubin, RA; Modrich, P

Published Date

  • August 1, 1978

Published In

Volume / Issue

  • 5 / 8

Start / End Page

  • 2991 - 2997

PubMed ID

  • 211492

Pubmed Central ID

  • 211492

International Standard Serial Number (ISSN)

  • 0305-1048

Digital Object Identifier (DOI)

  • 10.1093/nar/5.8.2991

Language

  • eng

Conference Location

  • England