Enzymatic characterization of a mutant of Escherichia coli with an altered DNA ligase.

Published

Journal Article

A temperature-sensitive, radiation-sensitive mutant of Escherichia coli has been assayed for DNA ligase activity in vitro. The strain contains a markedly reduced amount of DNA-joining activity, which is thermolabile. The formation of the ligase-adenylate intermediate is also temperature-sensitive in vitro. Two temperature-resistant revertants of the mutant contain normal amounts of a thermostable ligase. The mutant is killed by growth at 42 degrees C, a temperature at which it displays aberrant DNA synthesis. These results suggest that the ligase is necessary for normal DNA metabolism and viability in this strain.

Full Text

Duke Authors

Cited Authors

  • Modrich, P; Lehman, IR

Published Date

  • May 1, 1971

Published In

Volume / Issue

  • 68 / 5

Start / End Page

  • 1002 - 1005

PubMed ID

  • 4995816

Pubmed Central ID

  • 4995816

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.68.5.1002

Language

  • eng

Conference Location

  • United States